https://scholars.lib.ntu.edu.tw/handle/123456789/193990
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 臺大醫學院-泌尿科; | - |
dc.contributor.author | Wu, Chien-Ming | en |
dc.contributor.author | Lin, Kai-Wei | en |
dc.contributor.author | Teng, Chi-Hung | en |
dc.contributor.author | Huang, A-Mei | en |
dc.contributor.author | Chen, Yu-Chian | en |
dc.contributor.author | Yen, Ming-Hong | en |
dc.contributor.author | Wu, Wen-Bin | en |
dc.contributor.author | Pu, Yeong-Shiau | en |
dc.contributor.author | Lin, Chun-Nan | en |
dc.creator | Wu, Chien-Ming;Lin, Kai-Wei;Teng, Chi-Hung;Huang, A-Mei;Chen, Yu-Chian;Yen, Ming-Hong;Wu, Wen-Bin;Pu, Yeong-Shiau;Lin, Chun-Nan | en |
dc.creator | 蒲永孝 | zh-tw |
dc.date | 2014 | - |
dc.date.accessioned | 2017-09-27T04:10:49Z | - |
dc.date.accessioned | 2018-07-11T17:22:46Z | - |
dc.date.available | 2017-09-27T04:10:49Z | - |
dc.date.available | 2018-07-11T17:22:46Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/283538 | - |
dc.description.abstract | In an effort to develop potent cyclooxygenase-1 (COX-1) inhibitors used as anticancer agent, a series of 2',5'-dimethoxychalcones was screened to evaluate their antiplatelet effect on human washed platelets suspension. Compound 2 exhibited potent inhibition of human washed platelet aggregation induced by collagen, significantly inhibited collagen- and arachidonic acid-induced thromboxane B, release, and revealed inhibitory effect on COX-1 activity. Molecular docking studies showed that 1, 2, and 4 were bound in the active site of COX-1. These indicated that the antiplatelet effect of these compounds were mainly mediated through the suppression of COX-1 activity and reduced the thromboxane formation. To investigate the mechanistic action of COX-1 inhibitor enhanced the cytotoxic effect against human bladder cancer cells, NTUB1, we assessed the cytotoxic effect of 2 against NTUB1. Treatment of NTUB1 cells with various concentrations of 2 led to a concentration-dependent increase of cell death and decrease of reactive oxygen species levels. The flow-cytometric analysis showed that 2 induced a G1 phase cell cycle arrest but did not accompany an appreciable sub-G1 phase in NTUB1 cells. In addition, compound 2 increased p21 and p27 expressions and did not inhibit the expression of COX-1 in NTUB1 cells. Our results suggested that 2 enhanced cell growth inhibition or antiproliferative activity in NTUB1 cells through G1 arrest by COX-1 independent mechanism. | - |
dc.language | en-us | - |
dc.relation | Biol. Pharm. Bull., 37(7), 1191-1198 | - |
dc.relation.ispartof | Biol. Pharm. Bull. | - |
dc.subject | antiplatelet | - |
dc.subject | chalcone | - |
dc.subject | cyclooxygenase-1 (COX-1) | - |
dc.subject | G1 cell cycle arrest | - |
dc.subject | cytotoxicity | - |
dc.subject | bladder cancer | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | Chalcone Derivatives Inhibit Human Platelet Aggregation and Inhibit Growth in Human Bladder Cancer Cells | - |
dc.relation.pages | 1191-1198 | - |
dc.relation.journalvolume | 37 | - |
dc.relation.journalissue | 7 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。