https://scholars.lib.ntu.edu.tw/handle/123456789/194582
標題: | 嚴重急性呼吸道症候群的免疫致病機轉─(子計畫四)SARS病人抗肺部組織及細胞自體抗體之偵測 | 作者: | 楊曜旭 | 關鍵字: | 嚴重急性呼吸道症候群;自體抗體;內皮細胞;上皮細胞;細胞毒殺;SARS;autoantibodies;epithelial cell;endothelial cell;cytotoxicity | 公開日期: | 2004 | 出版社: | 臺北市:國立臺灣大學醫學院小兒科 | 摘要: | 嚴重急性呼吸道症候群 (SARS)是一種由SARS-associated 冠狀病毒感染而引起 肺部嚴重發炎,甚至纖維化的非典型性肺炎。在這個研究中,我們將探討感染此 冠狀病毒後身體是否會產生抗血管內皮細胞及肺部上皮細胞之自體抗體。血清來 自病人疾病不同時期(phase I, phase II/III)及健康對照組。目標細胞我們選用1) A549 human pulmonary epithelial cell-line,2) human umbilical venous endothelial cells (HUVEC),3) primary human pulmonary endothelial cells (HPEC)。自體抗體 的偵測是採用cell-based ELISA,indirect immunofluorescence staining,及flow cytometry 三種方法。結果顯示不管用那一種方法,病人於phase II/III 的血清中 存IgG anti-A549 cells antibodies,IgG anti-HUVEC antibodies,及IgM anti-HPEC antibodies 三種自體抗體。進一步的研究顯示IgG anti-A549 cells 自體抗體具有 complement-dependent 細胞毒殺作用。本實驗結論:感染SARS-associated 冠狀病 毒後期,病人血清會產生抗內皮細胞及上皮細胞之自體抗體,而這些感染後產生 的自體抗體所扮演的角色則須要更多的研究來確定。 The severe acute respiratory syndrome (SARS), an atypical pneumonia emerged in 21st century, is caused by the invasion of the SARS-associated coronavirus (SARS-CoV) and characterized by severe pulmonary inflammation and fibrosis. In this study, we investigate the possibilities of the development of autoantibodies against human epithelial cells and endothelial cells in patients with SARS at different time periods (the first week: phase I, 1-2 months after the disease onset: phase II/phase III). Antibodies in sera of patients and normal healthy controls against 1) A549 human pulmonary epithelial cell-line, 2) human umbilical venous endothelial cells (HUVEC), 3) primary human pulmonary endothelial cells (HPEC) were detected by the methods of cell-based ELISA, indirect immunofluorescence staining, and flow cytometry. The results of ELISA revealed that serum levels of IgG anti-A549 cells antibodies, IgG anti-HUVEC antibodies, and IgM anti-HPEC antibodies were significantly higher in SARS patients at phase II/phase III than those in healthy controls. Results of the other two tests, indirect immunofluorescence staining and flow cytometry, were consistent with the previous one. Sera from SARS patients at phase II/phase III could mediate complement dependent cytotoxicity against A549 cells. It is concluded that some autoantibodies against human epithelial cells and endothelial cells would be developed after SARS-CoV infection and this phenomenon may indicate the post-infectious cellular injury and also provide the possibility of SARS-induced immunopathology. III |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/29836 | 其他識別: | 922751B002011Y | Rights: | 國立臺灣大學醫學院小兒科 |
顯示於: | 醫學系 |
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