https://scholars.lib.ntu.edu.tw/handle/123456789/194952
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 小兒科 | en |
dc.contributor.author | CHANG, LUAN-YIN | en |
dc.creator | 張鑾英 | zh-tw |
dc.creator | CHANG, LUAN-YIN | en |
dc.date | 2002 | en |
dc.date.accessioned | 2008-12-29T06:14:11Z | - |
dc.date.accessioned | 2018-07-11T17:55:27Z | - |
dc.date.available | 2008-12-29T06:14:11Z | - |
dc.date.available | 2018-07-11T17:55:27Z | - |
dc.date.issued | 2002 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/94844 | - |
dc.description.abstract | Hand, foot and mouth disease is a common illness in children and is usually caused by coxsackievirus A 16 and enterovirus 71. It has been noted that enterovirus 71 infection is more severe with significantly greater frequency of serious complications and fatality than coxsackievirus A 16. Therefore, it is important to develop a rapid and specific assay for discriminating coxsackievirus A 16 and enterovirus 71 in hand, foot and mouth disease outbreaks. In this study we designed two sets of RT-PCR primers specific for coxsackievirus A 16 and enterovirus 71 . One hundred and eighty-nine viruses were evaluated for this molecular diagnosis assay. Among 110 enterovirus 71 strains , the enterovirus 71 specific primers gave clear signal for 107 clinical enterovirus 71 isolates and three reference enterovirus 71 strains. None of coxsackievirus A 16, other enteroviruses or non-enteroviruses show signal for enterovirus 71-specific primers. On the other hand, among 28 coxsackievirus A 16 strains, the coxsackievirus A 16- specific primers detect 27 clinical isolates and one reference strain but show no cross- reaction with other viruses. The molecular assay developed in this study provides a sensitive and specific way to distinguish coxsackievirus A 16 and enterovirus 71 induced hand, foot and mouth disease, which will be a useful rapid diagnostic method in future outbreaks. | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | JOURNAL OF VIROLOGICAL METHODS v.102 n.1-2 pp.9-14 | en |
dc.relation.ispartof | JOURNAL OF | - |
dc.subject | Hand | en |
dc.subject | foot and mouth disease | en |
dc.subject | Coxsackievirus A 16 | en |
dc.subject | Enterovir | en |
dc.title | Use of Molecular Assay in Diagnosis of Hand, Foot and Mouth Disease Caused by Enterovirus 71 or Coxsackievirus a 16 | en |
dc.type | journal article | en |
dc.relation.pages | 9-14 | - |
dc.relation.journalvolume | VIROLOGICAL | - |
dc.relation.journalissue | n.1-2 | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en_US | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
顯示於: | 醫學系 |
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