https://scholars.lib.ntu.edu.tw/handle/123456789/194963
Title: | Antitumor and Antimetastatic Activity of Il-23 | Authors: | Lo, C.-H. Lee, S.-C. Wu, P.-Y. Pan, W.-Y. Su, J. Cheng, C.-W. Roffler, S. R. Chiang, B.-L. Lee, C.-N. Wu, C.-W. Tao, M.-H. |
Keywords: | IFN-GAMMA PRODUCTION;TUMOR-REGRESSION;IMMUNE-RESPONSE;COLON- TUMOR;T-CELLS;INTERLEUKIN-12 | Issue Date: | 2003 | Journal Volume: | v.171 | Journal Issue: | n.2 | Start page/Pages: | 600-607 | Source: | JOURNAL OF IMMUNOLOGY | Abstract: | The structure and T cell stimulatory effects of the recently discovered cytokine IL-23 are similar to, but distinct from , those of IL-12. Although the antitumor activities of IL-12 are well characterized, the effect of IL-23 on tumor growth is not known. In this study, murine CT26 colon adenocarcinoma and B16F1 melanoma cells were engineered using retroviral vectors to release single-chain IL-23 (scIL -23) to evaluate its antitumor activity. In BALB/c mice, scIL-23-transduced CT26 cells grew progressively until day 26 to an average size of 521 +/- 333 mm(3), then the tumors started to regress in most animals, resulting in a final 70% rate of complete tumor rejection. scIL-23 transduction also significantly suppressed lung metastases of CT26 and B16F1 tumor cells. In addition, mice that rejected scIL-23- transduced tumors developed a memory response against subsequent wild-type tumor challenge. Compared with scIL-12- expressing CT26 cells, scIL-23-transduced tumors lacked the early response , but achieved comparable antitumor and antimetastatic activity. These results demonstrated that IL- 23, like IL-12, provided effective protection against malignant diseases, but it probably acted by different antitumor mechanisms. As a first step in identifying these antitumor mechanisms, tumor challenge studies were performed in immunocompromised hosts and in animals selectively depleted of various lymphocyte populations. The results showed that CD8(+) T cells, but not CD4(+) T cells or NK cells, were crucial for the antitumor activity of IL-23. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94855 |
Appears in Collections: | 醫學系 |
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