https://scholars.lib.ntu.edu.tw/handle/123456789/197821
標題: | Hypoxia-Mediated down-Regulation of Octn2 and Ppar Alpha Expression in Human Placentas and in Bewo Cells | 作者: | CHANG, TING-TING SHYU, MING-KWANG HUANG, MIN-CHUAN HSU, CHEN-CHI YEH, SZU-YU CHEN, MEI-RU LIN, CHUN-JUNG |
關鍵字: | OCTN2;PPAR alpha;Preeclampsia;Hypoxia;BeWo cells | 公開日期: | 2011 | 卷: | v.8 | 期: | n.1 | 起(迄)頁: | 117-125 | 來源出版物: | MOLECULAR PHARMACEUTICS | 摘要: | The objective of the present study was to investigate the effects of hypoxia on placental expression of OCTN2 and PPAR alpha. OCTN2 and PPAR alpha expression in the human placenta in the presence or absence of preeclampsia was examined by immunohistochemical IHC analysis, Western blotting, and quantitative polymerase chain reaction qPCR. Effects of hypoxia on the expression of OCTN2 and PPAR alpha in human placental explants and human choriocarcinoma BeWo cells were examined by Western blotting and qPCR analyses. IHC, Western blot, and qPCR studies showed that OCTN2 and PPAR alpha protein and mRNA levels were lower in syncytiotrophoblasts from preeclamptic human placentas than in those from normal placentas. Hypoxic treatment caused a decrease in OCTN2 and PPAR alpha expression in human placental explants and in BeWo cells. VVY14643, a PPAR alpha agonist, caused an increase in OCTN2 expression in BeWo cells under hypoxic conditions. In conclusion, under hypoxic conditions, placental OCTN2 is down-regulated through PPAR alpha-mediated pathways. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/241745 |
顯示於: | 醫學系 |
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