https://scholars.lib.ntu.edu.tw/handle/123456789/201991
標題: | Sars Antibody Test for Serosurveillance | 作者: | HSUEH, PO-REN KAO, CHUAN-LIANG LEE, CHUN-NAN HO, MEI-SHANG CHARLES SIA SHUGENE LYNN ALAN M. WAIFIELD WANG, CHANG-YI |
公開日期: | 2004 | 卷: | v.10 | 期: | n.9 | 起(迄)頁: | 1558-1562 | 來源出版物: | EMERGING INFECTIOUS DISEASES | 摘要: | A peptide-based enzyme-linked immunosorbent assay (ELISA) can be used for retrospective serosurveillance of severe acute respiratory syndrome (SARS ) by helping identify undetected chains of disease transmission. The assay was developed by epitope mapping, using synthetic peptides from the spike , membrane, and nucleocapsid protein sequences of SARS-associated coronavirus. The new peptide ELISA consistently detected seroconversion by week 2 of onset of fever, and seropositivity remained through day 100. Specificity was 100% on normal blood donor samples, on serum samples associated with infection by other pathogens, and on an interference panel . The peptide-based test has advantages of safety, standardization, and automation over previous immunoassays for SARS. The assay was used for a retrospective survey of healthy healthcare workers in Taiwan who treated SARS patients. Asymptomatic seroconversions were detected in two hospitals that had nosocomial disease. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/89438 |
顯示於: | 醫學系 |
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