https://scholars.lib.ntu.edu.tw/handle/123456789/202191
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 檢驗醫學科 | en |
dc.contributor.author | Chang, Yu-Jia | en |
dc.contributor.author | Wu, Hua-Lin | en |
dc.contributor.author | Hsu, Ya-Chu | en |
dc.contributor.author | Hamaguchi, Nobuko | en |
dc.contributor.author | Shi, Guey-Yueh | en |
dc.contributor.author | Shen, Ming-Ching | en |
dc.contributor.author | Lin, Shu-Wha | en |
dc.creator | 許雅筑;沈銘鏡;林淑華 | zh-tw |
dc.creator | Chang, Yu-Jia; Wu, Hua-Lin; Hsu, Ya-Chu; Hamaguchi, Nobuko; Shi, Guey-Yueh; Shen, Ming-Ching; Lin, Shu-Wha | en |
dc.date | 2003 | en |
dc.date.accessioned | 2009-01-20T06:41:48Z | - |
dc.date.accessioned | 2018-07-12T09:14:21Z | - |
dc.date.available | 2009-01-20T06:41:48Z | - |
dc.date.available | 2018-07-12T09:14:21Z | - |
dc.date.issued | 2003 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/103381 | - |
dc.description.abstract | Anti-human factor IX monoclonal antibody, A-5 (Mab A-5), has been widely used in structure-function studies for factor IX. Mab A-5 recognizes the catalytic domain of human factor IX (FIX). Regions important for Mab A-5 binding have previously been localized to the amino terminus of the heavy chain of factor IX, encompassing amino acid residues 181- 310 [Blood (74) 971]. We have selected 20 positions in this region for alanine-scanning mutagenesis. We found that Mab A -5 failed to react with the recombinant factor IX mutants with substitutions at positions 228 and 252. Mab A-5 also reacted to a lesser extent to FIXD276A (factor IX with alanine substitution for aspartic acid at residue 276) and FIXK201A/D203A (double alanine substitutions at residues 201 and 203). The apparent dissociation rate constants (K-D) in binding Mab A-5 were 6.0 x 10(-9), 1.4 x 10(-8) and 2.0 x 10(-8) M, for wild-type FIX, FIXK201A/D203A and FIXD276A, respectively. The increased K-D values of the two FIX mutants are mainly owing to the increased dissociation rates . These affected residues constitute a surface opposite from the factor VIIIa binding surface. We conclude that the epitope for Mab A-5 is on a surface composed of residues 228 , 252, 276, and 201 or 203. This surface, which may not be important for factor VIII binding, contains a strong antigenic region on factor IX. (C) 2003 Elsevier Ltd. All rights reserved | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | THROMBOSIS RESEARCH v.111 n.4-5 pp.293-299 | en |
dc.relation.ispartof | THROMBOSIS RESEARCH | - |
dc.subject | factor IX | en |
dc.subject | alanine-scanning mutagenesis | en |
dc.subject | recombinant proteins | en |
dc.subject | monoclonal antibody | en |
dc.subject | conformational epitope | en |
dc.subject | surface plasmon resonance | en |
dc.title | Discontinuous Residues of Factor Ix Constitute a Surface for Binding the Anti-Factor Ix Monoclonal Antibody a-5 | en |
dc.relation.pages | 293-299 | - |
dc.relation.journalvolume | v.111 | - |
dc.relation.journalissue | n.4-5 | - |
item.languageiso639-1 | en_US | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
顯示於: | 醫學系 |
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