https://scholars.lib.ntu.edu.tw/handle/123456789/204716
標題: | Rhodostomin Inhibits the Transforming Growth Factor-Beta1- Enhanced Adhesion | 作者: | YANG, RONG-SEN HUANG, TUR-FU |
公開日期: | 2000 | 卷: | v.191(3) | 起(迄)頁: | 145-55 | 來源出版物: | TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE | 摘要: | We have investigated the effect of transforming growth factor-beta1 (TGF- beta1) on the in vitro adhesion activity of the rat osteosarcoma cell lines (ROS 17/2.8) to extracellular matrix substrata, including fibronectin, type I and IV collagen, as well as laminin. The interaction of Arg-Gly-Asp (RGD) and rhodostomin, an RGD containing snake venom, with TGF-beta1 on the cell adhesion was also evaluated. The results showed that incubation with various concentration of TGF-beta1 (1-15 ng/ml) significantly increased the adhesion activity (1.4 to 2.5 folds) of ROS 17 /2.8 to fibronectin and type I collagen (p<0.01), whereas the adhesion activity to laminin and type IV collagen was slightly elevated (1.1 to 1.5 folds). The peak effect of TGF -beta1 on the cell adhesion occurred after pretreatment of ROS 17/2.8 with TGF-beta1 for 6 hours. Treatment with Arg - Gly-Asp-Ser (RGDS) and rhodostomin effectively suppressed the TGF-beta1- enhanced adhesion activity to fibronectin and type I collagen. This study demonstrated that the up- regulated cell adhesion activity of ROS 17/2.8 cells by the TGF-beta1 can be inhibited by the rhodostomin. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/92388 |
顯示於: | 醫學系 |
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