https://scholars.lib.ntu.edu.tw/handle/123456789/204746
標題: | Coculture of Endothelial and Smooth Muscle Cells on a Collagen Membrane in the Development of a Small-Diameter Vascular Graft | 作者: | WU, HSI-CHIN WANG, TZU-WEI KANG, PEI- LEUN TSUANG, YANG-HWEI SUN, JUI-SHENG LIN, FENG-HUEI |
關鍵字: | biomimetic material;collagen;co-culture;vascular grafts | 公開日期: | 2007 | 卷: | v.28 | 期: | n.7 | 起(迄)頁: | 1385-1392 | 來源出版物: | BIOMATERIALS | 摘要: | In this study, we have evaluated the feasibility of developing a biodegradable collagenous small diameter vascular graft of 2mm diameter and 1 cm length. In brief, bi -layer type I collagen membrane was fabricated under vacuum suction and lyophilization methods. The smooth muscle cells were inoculated into the lower side of the porous membrane, while endothelial cells were seeded onto upper smooth side of the membrane . After cultured for 7 days, the vascular substitute was either harvested for in vitro examination or in vivo implanted in the subcutaneous layer for biocompatibility test. The tubular vascular prosthesis was then used as a temporary absorbable guide that served as an in vivo vascular graft to promote the complete regeneration of rat inferior vena cava. After implantation for 12 weeks, a thin continuous layer of endothelial cells and smooth muscle cells were lined with the vascular lumen and tunic media , respectively. Histology results showed that there were no signs of significant thrombogeneity and intima hyperplasia. This tissue engineered vascular substitute not only had enough tensile strength and good biocompatibility, but also advanced vascular regeneration. In the future, we suggest that this biodegradable vascular substitute will provide with the possibility in application on small diameter prosthetic grafts in artificial blood vessels. (c) 2006 Elsevier Ltd. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/92429 |
顯示於: | 醫學系 |
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