https://scholars.lib.ntu.edu.tw/handle/123456789/292213
標題: | Use of bacterially expressed EBNA-1 protein cloned from a nasopharyngeal carcinoma (NPC) biopsy as a screening test for NPC patients | 作者: | MEI-RU CHEN Liu, Mei-Ying Hsu, Shih-Mei Fong, Chin-Chu Chen, Chien-Jen Chen, I-How Hsu, Mow-Ming Yang, Czau-Siung Chen, Jen-Yang |
關鍵字: | Antibodies; EBNA-1; NPC | 公開日期: | 2001 | 卷: | 64 | 期: | 1 | 起(迄)頁: | 51-57 | 來源出版物: | Journal of Medical Virology | 摘要: | EBV serological tests have been used for many years as accessory diagnostic predictors of nasopharyngeal carcinoma (NPC). To increase the sensitivity and specificity of the NPC detection rate, a novel enzyme-linked immunosorbent assay (ELISA) was established using a bacterially-expressed GST-EBNA-1 protein, containing the EBNA-1 sequence cloned from an NPC patient. Serum samples were collected from age- and gender-matched patients with NPC, community control subjects and hospital control patients and tested using this ELISA. The positivity rates were 78.7% (247/314) in NPC, 11.5% (28/244) in hospital controls and 3.8% (10/263) in the community control group. These serum samples were also tested for IgA anti-VCA antibodies and their ability to neutralize EBV DNase and the sensitivities of the anti-VCA antibody and DNase-neutralization tests also were analyzed. The optimum combination is VCA plus EBNA-1, which can identify 92.5% (287/310) of NPC patients, and shows a specificity of 92.7% (242/261) for normal individuals. ? 2001 Wiley-Liss, Inc. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0035085293&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/292213 |
DOI: | 10.1002/jmv.1017 | SDG/關鍵字: | cell nucleus antigen; deoxyribonuclease; EBNA 1 protein; Epstein Barr virus antigen; glutathione transferase; unclassified drug; virus capsid antigen; article; cancer diagnosis; cancer screening; controlled study; diagnostic accuracy; diagnostic procedure; enzyme linked immunosorbent assay; Epstein Barr virus; human; nasopharynx carcinoma; screening test; serodiagnosis; Antibodies, Viral; Antigens, Viral; Biopsy; Capsid Proteins; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Epitopes; Epstein-Barr Virus Nuclear Antigens; Escherichia coli; Humans; Immunoglobulin A; Matched-Pair Analysis; Nasopharyngeal Neoplasms; Neutralization Tests; Plasmids; Recombinant Fusion Proteins; Sensitivity and Specificity; Serologic Tests; Taiwan |
顯示於: | 醫學系 |
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