https://scholars.lib.ntu.edu.tw/handle/123456789/297750
標題: | Possible involvement of glutathione and p53 in trichloroethylene- and perchloroethylene-induced lipid peroxidation and apoptosis in human lung cancer cells | 作者: | Chen, S.-J. Wang, J.-L. Chen, J.-H. Huang, R.-N. RONG-NAN HUANG |
關鍵字: | Apoptosis; Free radicals; Glutathione; Lipid peroxidation; p53; Perchloroethylene; Trichloroethylene | 公開日期: | 2002 | 卷: | 33 | 期: | 4 | 起(迄)頁: | 464-472 | 來源出版物: | Free Radical Biology and Medicine | 摘要: | Trichloroethylene (TCE) and perchloroethylene (PERC) are volatile organic compounds (VOCs) that are primarily inhaled through the respiratory system. The aim of this study was to elucidate the role of glutathione (GSH) and p53 in TCE- and PERC-induced lung toxicity. Human lung adenocarcinoma cells NCI-H460 (p53-wild-type) have constitutively lower levels of GSH than NCI-H1299 (p53-null) cells. The results showed that exposure to vapor TCE and PERC produced a dose-dependent and more pronounced accumulation of H2O2 in p53-WT H460 than p53-null H1299 cells. The accumulation of H2O2 was accompanied by severe cellular damage, as indicated by the significant increase of lipid peroxidation and apoptosis in p53-WT H460 cells, but not p53-null H1299 cells. Cotreatment of p53-WT H460 cells with free radical scavengers, such as D-mannitol, uric acid, and sodium selenite, significantly attenuated the TCE- or PERC-induced lipid peroxidation. In contrast, depletion of GSH in p53-null H1299 cells enhanced TCE- or PERC-induced lipid peroxidation. The levels of p53 and Baxproteins were elevated, while Bcl-2 protein was downregulated in TCE- or PERC-treated p53-WT H460 cells. Activity of caspase 3, the apoptotic executioner, was also significantly enhanced in TCE- or PERC-treated cells. These data suggest that, in human lung cancer cells, GSH plays a vital role in the protection of TCE- and PERC-induced oxidative stress and apoptosis, which may be mediated through a p53-dependent pathway. ? 2002 Elsevier Science Inc. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0037101880&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/297750 |
DOI: | 10.1016/S0891-5849(02)00817-1 | SDG/關鍵字: | glutathione peroxidase; hydrogen peroxide; mannitol; protein Bax; protein bcl 2; protein p53; scavenger; sodium selenite; tetrachloroethylene; trichloroethylene; uric acid; volatile organic compound; BAX protein, human; CASP3 protein, human; caspase; caspase 3; glutathione; oncoprotein; protein Bax; protein bcl 2; protein p53; reactive oxygen metabolite; thiobarbituric acid reactive substance; tumor protein; apoptosis; article; cancer cell; cell damage; cell line; controlled study; dose response; down regulation; human; human cell; inhalation; lipid peroxidation; lung adenocarcinoma; lung cancer; lung toxicity; oxidative stress; priority journal; respiratory system; adenocarcinoma; biosynthesis; cell culture; comparative study; drug effect; enzyme activation; gas; gene expression regulation; lung tumor; metabolism; pathology; physiology; Adenocarcinoma; Apoptosis; Caspases; Comparative Study; Enzyme Activation; Free Radical Scavengers; Gases; Gene Expression Regulation, Neoplastic; Glutathione; Human; Hydrogen Peroxide; Lipid Peroxidation; Lung Neoplasms; Mannitol; Neoplasm Proteins; Oxidative Stress; Protein p53; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-bcl-2; Reactive Oxygen Species; Sodium Selenite; Support, Non-U.S. Gov't; Tetrachloroethylene; Thiobarbituric Acid Reactive Substances; Trichloroethylene; Tumor Cells, Cultured; Uric Acid; bcl-2-Associated X Protein; Caspase 3; Caspases; Enzyme Activation; Free Radical Scavengers; Gases; Gene Expression Regulation, Neoplastic; Humans; Hydrogen Peroxide; Mannitol; Oxidative Stress; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-bcl-2; Reactive Oxygen Species; Sodium Selenite; Thiobarbituric Acid Reactive Substances; Tumor Cells, Cultured; Tumor Suppressor Protein p53; Uric Acid; Lethrinidae |
顯示於: | 昆蟲學系 |
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