|Title:||The effect of an RGD-containing fusion protein CBD-RGD in promoting cellular adhesion||Authors:||Hsu, S.-H.
|Keywords:||RGD-containing peptides;polyurethane;poly(L-lactide);poly( lactide-co-glycolide)||Issue Date:||2004||Journal Volume:||111||Journal Issue:||2||Start page/Pages:||143-154||Source:||Journal of Biotechnology||Abstract:||
The effect of a recombinant RGD (arginine-glycine-aspartic acid)- containing fusion protein, cellulose-binding domain ( CBD)RGD, on the cellular adhesion to a biomedical polyurethane (PU) was evaluated. A series of different cell lines, as well as freshly harvested animal cells, were grown on the PU surfaces with or without CBD-RGD, in serum or serum- free media. The results showed that the enhancement of cellular attachment by CBD-RGD varied with cell types. This is believed to be a result of the unique integrin receptors on each type of cell surface. The existence of certain divalent ions (Mg2+ and Mn2+) may increase the efficacy of the CBD -RGD, in a cell type-dependent manner. The fusion protein was also found to inhibit the platelet activation. The effect of CBD-RGD was further examined in two other substrate materials, poly(L-lactide) (PLLA) and poly (lactide -co-glycolide) (PLGA). The effect on cellular adhesion correlated with the amount of CBD-RGD physically adsorbed on the material surface. ( C) 2004 Elsevier B.V. All rights reserved.
|Appears in Collections:||高分子科學與工程學研究所|
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