https://scholars.lib.ntu.edu.tw/handle/123456789/307082
Title: | The effect of an RGD-containing fusion protein CBD-RGD in promoting cellular adhesion | Authors: | Hsu, S.-H. Chu, W.-P. Lin, Y.-S. Chiang, Y.-L. Chen, D.C. Tsai, C.-L. SHAN-HUI HSU 江郁霖 陳震漢 蔡清霖 |
Keywords: | RGD-containing peptides;polyurethane;poly(L-lactide);poly( lactide-co-glycolide) | Issue Date: | 2004 | Journal Volume: | 111 | Journal Issue: | 2 | Start page/Pages: | 143-154 | Source: | Journal of Biotechnology | Abstract: | The effect of a recombinant RGD (arginine-glycine-aspartic acid)- containing fusion protein, cellulose-binding domain ( CBD)RGD, on the cellular adhesion to a biomedical polyurethane (PU) was evaluated. A series of different cell lines, as well as freshly harvested animal cells, were grown on the PU surfaces with or without CBD-RGD, in serum or serum- free media. The results showed that the enhancement of cellular attachment by CBD-RGD varied with cell types. This is believed to be a result of the unique integrin receptors on each type of cell surface. The existence of certain divalent ions (Mg2+ and Mn2+) may increase the efficacy of the CBD -RGD, in a cell type-dependent manner. The fusion protein was also found to inhibit the platelet activation. The effect of CBD-RGD was further examined in two other substrate materials, poly(L-lactide) (PLLA) and poly (lactide -co-glycolide) (PLGA). The effect on cellular adhesion correlated with the amount of CBD-RGD physically adsorbed on the material surface. ( C) 2004 Elsevier B.V. All rights reserved. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-3042519184&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/307082 |
DOI: | 10.1016/j.jbiotec.2004.03.014 |
Appears in Collections: | 高分子科學與工程學研究所 |
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