https://scholars.lib.ntu.edu.tw/handle/123456789/307768
Title: | Distinct Effects on Gene Expression of Chemical and Genetic Manipulation of the Cancer Epigenome Revealed by a Multimodality Approach | Authors: | ERIC YAO-YU CHUANG | Issue Date: | Oct-2004 | Source: | Cancer Cell | Abstract: | We tested the hypothesis that the effects on gene expression of altered DNA methylation by 5-aza-2′-deoxycytidine (5-aza-CdR) and genetic (DNMT knockout) manipulation of DNA are similar, and distinct from Trichostatin A (TSA)-induced chromatin decondensation. Surprisingly, the effects of 5-aza-CdR were more similar to those of TSA than to DNMT1, DNMT3B, or double DNMT somatic cell knockout. Furthermore, the effects of 5-aza-CdR were similar at one and five days exposure, suggesting active demethylation or direct influence of both drugs on the stability of methylation and/or chromatin marks. Agents that induce gene activation through hypomethylation may have unintended consequences, since nearly as many genes were downregulated as upregulated after demethylation. In addition, a 75 kb cluster of metallothionein genes was coordinately regulated. |
URI: | http://www.ncbi.nlm.nih.gov/pubmed/15488759?dopt=Abstract https://www.scopus.com/inward/record.uri?eid=2-s2.0-5444227862&doi=10.1016%2fj.ccr.2004.08.029&partnerID=40&md5=dc6bce21fefeef74cf97407e90716035 |
ISSN: | 15356108 | DOI: | 10.1016/j.ccr.2004.08.029 | SDG/Keyword: | 5 aza 2' deoxycytidine; DNA methyltransferase 1; DNA methyltransferase 3B; metallothionein; trichostatin A; article; cancer genetics; chromatin; controlled study; demethylation; DNA methylation; down regulation; gene activation; gene expression; genetic manipulation; human; human cell; knockout gene; nucleotide sequence; polymerization; priority journal; somatic cell; upregulation |
Appears in Collections: | 生醫電子與資訊學研究所 |
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