|Title:||Genomic heterogeneity in Klebsiella pneumoniae strains is associated with primary pyogenic liver abscess and metastatic infection||Authors:||CHI-TAI FANG
|Issue Date:||2005||Journal Volume:||192||Journal Issue:||1||Start page/Pages:||117-128||Source:||Journal of Infectious Diseases||Abstract:||
Background. Primary pyogenic liver abscess (PLA) with septic complication by Klebsiella pneumoniae is an emerging infectious disease. Methods and results. Using DNA microarray hybridization, we identified a 20-kb chromosomal region that contained 15 open-reading frames (ORFs), including an iron-uptake system (kfu), a phosphoenolpyruvate sugar phosphotransferase system (PTS), and 6 unknown ORFs. The region was more prevalent among tissue-invasive strains (35/46) than among noninvasive strains (19/98) (P < .0001, χ2 test). To test the role played by this region in pathogenesis, 3 different deletion mutants (NTUH-K2044 [Δkfu], K2044 [ΔORF7-9], and K2044 [ΔPTS]) were constructed. Only the ΔkfuABC mutants showed decreased virulence in mice, compared with the wild-type strain. An in vitro assay confirmed the involvement of kfu in iron acquisition. There was a high correlation rate (85%) between the kfu/PTS region and 2 tissue invasion-associated chromosomal regions (allS and magA). Moreover, all 3 regions were present in strains that caused PLA plus endophthalmitis or meningitis. Conclusion. Our results suggest that chromosomal heterogeneity is present in tissue-invasive K. pneumoniae strains. A genotype containing all 3 regions is strongly associated with PLA and metastatic infection. These regions may serve as convenient markers for the rapid diagnosis of emergent tissue-invasive strains. ? 2005 by the Infectious Diseases Society of America. All rights reserved.
|DOI:||10.1086/430619||metadata.dc.subject.other:||amino acid sequence; animal experiment; animal model; animal tissue; article; bacterial chromosome; bacterial meningitis; bacterial strain; bacterial virulence; colorimetry; controlled study; deletion mutant; DNA hybridization; DNA microarray; endophthalmitis; genetic heterogeneity; genotype; Gram negative sepsis; histopathology; human; in vitro study; iron transport; Klebsiella pneumoniae; LD 50; liver abscess; mouse; nonhuman; nucleotide sequence; open reading frame; operon; plasmid; polymerase chain reaction; priority journal; promoter region; slot blot hybridization; strain difference; survival rate; wild type; Animals; Bacterial Proteins; Chromosome Mapping; Endophthalmitis; Genome, Bacterial; Humans; Klebsiella Infections; Klebsiella pneumoniae; Liver Abscess, Pyogenic; Meningitis, Bacterial; Mice; Mice, Inbred BALB C; Microarray Analysis; Plasmids; Sepsis; Variation (Genetics); Virulence
|Appears in Collections:||醫學系|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.