https://scholars.lib.ntu.edu.tw/handle/123456789/314907
標題: | Electrical detection of protein using gold nanoparticles and nanogap electrodes | 作者: | Tsai, C.-Y. Chang, T.-L. Uppala, R. Chen, C.-C. Fu-Hsiang, K.O. Chen, P.-H. PING-HEI CHEN |
關鍵字: | Electrical detection; Gold nanoparticle; Multilayer; Protein chip; Self-assembled monolayer | 公開日期: | 2005 | 卷: | 44 | 期: | 7 B | 起(迄)頁: | 5711-5716 | 來源出版物: | Japanese Journal of Applied Physics | 摘要: | A method of electrically detecting of protein described is developed using self-assembled multilayer gold nanoparticles (AuNPs) on a SiO2/Si substrate between gold electrodes. Electrical measurements are performed at room temperature using a probe station. A monoclonal antibody is immobilized on the top surface of the first layer of AuNPs (14nm). The second layer of AuNPs is formed through specific binding among a target antigen [hepatitis C virus, (HCV)], the monoclonal antibody, and the conjugate of a AuNP-polyclonal antibody. Once the specific binding among the monoclonal antibody, target antigen, and polyclonal antibody occurs, a significant electric current is detected through multilayer self-assembled gold nanoparticles between nanogap electrodes. No significant current (<1 pA) can be measured through a monolayer of AuNPs. A significant difference between the IV curves of the monolayer and the multilayer of AuNPs is used to identify whether the target antigen exists in the tested sample. ? 2005 The Japan Society of Applied Physics. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-31844448707&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/314907 |
DOI: | 10.1143/JJAP.44.5711 | SDG/關鍵字: | Gold; Monolayers; Nanostructured materials; Self assembly; Substrates; Electrical detection; Gold nanoparticles; Protein chip; Proteins |
顯示於: | 機械工程學系 |
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