https://scholars.lib.ntu.edu.tw/handle/123456789/321042
Title: | Proliferin enhances microvilli formation and cell growth of neuroblastoma cells | Authors: | Wang, Jyhi-Wai Jiang, Yan-Nian Huang, Ching-Ying Huang, Pei-Yu Huang, Mu-Chiou Cheng, Winston Teng-Kuei Shen, Che-Kun James YU-TEN JU |
Keywords: | IGF-II/M6P; Microvilli; Mitogen-regulated protein; Neuroblastoma; Proliferation; Proliferin | Issue Date: | 2006 | Journal Volume: | 56 | Journal Issue: | 1 | Start page/Pages: | 80-90 | Source: | Neuroscience Research | Abstract: | Proliferins (also termed mitogen-regulated proteins; MRP/PLFs) belong to the prolactin gene family. Mrp/Plfs are involved in angiogenesis of the uterus and placenta and maximally expressed during midgestation and decline through the remainder of the gestation period in mouse placenta. The tissue expressions of Mrp/Plfs are mainly documented in placenta, hair follicles of skin and in wound healing. In this report, we demonstrate that Plf1, Plf1 minus exon3, Plf2 and Mrp3 but not Mrp4 are expressed in mouse whole brain by diagnostic RT-PCR and Western blotting. The expression levels of Mrp/Plf mRNAs in mouse brains were low during the neonatal period, but higher in embryonic and adult stages, indicating Mrp/Plfs expression profiles are different in mouse brain and placenta. Interestingly, endogenous Mrp/Plfs were detected using immunostaining both in mouse brain sections and the neuroblastoma cell line, Neuro-2a cells. The function of PLF1 was explored by expressing exogenous PLF1 in Neuro-2a cells. This resulted in increased microvilli. Neuro-2a cells with stable expression of PLF1 had increased proliferation compared with normal and stable expressing EGFP cells when cell reached saturation density. Together these data, strongly suggest that MRP/PLFs mediate microvilli formation and contribute to cell proliferation of neuroblastoma cells. © 2006 Elsevier Ireland Ltd and the Japan Neuroscience Society. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-33747879542&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/321042 |
DOI: | 10.1016/j.neures.2006.05.011 | SDG/Keyword: | adult; animal cell; animal tissue; article; brain; brain region; cell function; cell growth; cell proliferation; comparative study; controlled study; developmental stage; embryo; embryo development; exon; female; immunohistochemistry; male; microvillus; mouse; neuroblastoma cell; newborn; newborn period; nonhuman; placenta; priority journal; protein analysis; protein expression; protein function; protein localization; reverse transcription polymerase chain reaction; Western blotting |
Appears in Collections: | 動物科學技術學系 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.