|Title:||Aqueous Nickel-Nitrilotriacetate Modified Fe3O4-NH3+ Nanoparticles for Protein Purification and Cell Targeting||Authors:||Dar-Bin Shieh
Jih Ru Hwu
|Issue Date:||Aug-2006||Journal Volume:||17||Journal Issue:||16||Start page/Pages:||4174-4182||Source:||Nanotechnology||Abstract:||
A comprehensive totally aqueous phase synthesis of nickel-nitrilotriacetate (Ni-NTA) modified superparamagnetic Fe3O4 nanoparticles is presented. The Fe3O4-NTA-Ni nanoparticles are able to perform efficient and specific purification of 6-His tagged proteins from crude cell lysates, as evidenced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. The average binding capacity, as demonstrated by streptopain (MW 42kDa), is 0.23mg/mg (protein/Fe3O4-NTA-Ni). Considering the high affinity and specificity of the binding between hexahistidine motif and Ni-NTA, Ni-NTA modified nanoparticles could act as a module to carry 6-His tagged proteins on the particle surface with molecular orientation control, since only the 6-His domain could be attached. These modularly designed functional nanoparticles enhance cancer cell targeting, as supported by the in vitro receptor mediated targeting assay using RGD-4C-6-His fusion peptide. The nanoparticles show no significant hemolysis for human blood and could be investigated further for their in vivo functional imaging applications. ? 2006 IOP Publishing Ltd.
|URI:||http://scholars.lib.ntu.edu.tw/handle/123456789/325449||DOI:||10.1088/0957-4484/17/16/030||metadata.dc.subject.other:||Blood; Iron compounds; Molecular orientation; Nickel; Polypeptides; Proteins; Hemolysis; Nanoparticles; Nickel-nitrilotriacetate (Ni-NTA); Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE); Nanostructured materials
|Appears in Collections:||電機工程學系|
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