https://scholars.lib.ntu.edu.tw/handle/123456789/329912
標題: | An apoptosis-related gene network induced by novel compound-cRGD in human breast cancer cells. | 作者: | Huang, Hsuan-Cheng Chang, Chih-Chin Chang, Hsin-Yi Ou, Chern-Han Hsu, Chun-Hua Chen, Shui-Tein Juan, Hsueh-Fen Huang, Tsui-Chin |
關鍵字: | Apoptosis; Caspase; cRGD; Integrin; MCF-7 | 公開日期: | 七月-2007 | 卷: | 581 | 期: | 18 | 起(迄)頁: | 3517-3522 | 來源出版物: | FEBS Letters | 摘要: | Synthetic peptides with the arginine-glycine-aspartate (RGD) motif have been used widely as inhibitors of integrin-ligand interactions to study cell growth, adhesion, migration and differentiation. We designed cyclic-RGD peptide (Tpa-RGDWPC-, cRGD) which could cause cell death in MCF-7 cell line. In order to understand the mechanism involved in cRGD-induced apoptosis, we used microarray, real-time quantitative PCR (Q-PCR) and bioinformatics to study the effects of cRGD on breast cancer cell line MCF-7. By time-series gene expression measurements and our developed software BSIP and GeneNetwork, we reconstructed an apoptosis-related gene network. In the network, caspase-9, located at the upstream, activates downstream effector caspases such as caspase-7, leading to the induction of caspase-4. Combined our previous proteomics data with newly performed docking simulation, it indicated that the cell death induced by cRGD may be triggered through blocking integrin signaling to the extracellular matrix and activation of caspase pathway. This study provides a molecular explanation of cRGD treatment in breast cancer cells and set forth a constructive far-reaching impact on breast cancer therapy. ? 2007 Federation of European Biochemical Societies. |
URI: | http://europepmc.org/abstract/med/17624335 http://scholars.lib.ntu.edu.tw/handle/123456789/329912 |
DOI: | 10.1016/j.febslet.2007.06.067 | SDG/關鍵字: | caspase 4; caspase 7; caspase 9; peptide; apoptosis; article; bioinformatics; breast cancer; cancer cell culture; cancer therapy; cell line; computer program; enzyme activation; extracellular matrix; gene expression; human; human cell; nucleotide sequence; priority journal; proteomics; reverse transcription polymerase chain reaction; signal transduction; Apoptosis; Breast Neoplasms; Caspases; Cell Line, Tumor; Computer Simulation; Gene Expression Regulation; Gene Regulatory Networks; Humans; Hydrophobicity; Ligands; Models, Molecular; Molecular Structure; Oligonucleotide Array Sequence Analysis; Peptides, Cyclic |
顯示於: | 生命科學系 |
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