https://scholars.lib.ntu.edu.tw/handle/123456789/332816
標題: | Gene expression profiling of breast, prostate, and glioma cells following single versus fractionated doses of radiation | 作者: | MONG-HSUN TSAI Cook, John A. ramouli, Gadisetti V.R. DeGraff, William Yan, Hailing Zhao, Shuping Coleman, C. Norman Mitchell, James B. ERIC YAO-YU CHUANG |
公開日期: | 2007 | 卷: | 67 | 期: | 8 | 起(迄)頁: | 3845-3852 | 來源出版物: | Cancer Research | 摘要: | Studies were conducted to determine whether gene expression profiles following a single dose of radiation would yield equivalent profiles following fractionated radiation in different tumor cell lines. MCF7 (breast), DU145 (prostate), and SF539 (gliosarcoma) cells were exposed to a total radiation dose of 10 Gy administered as a single dose (SD) or by daily multifractions (MF) of 5 x 2 Gy. Following radiation treatment, mRNA was isolated at 1, 4, 10, and 24 h and processed for cDNA microarray analysis. To determine the influence of the tumor microenvironment on gene expression, one cell type (DU145) was evaluated growing as a solid tumor in athymic nude mice for both radiation protocols. Unsupervised hierarchical cluster map analysis showed significant differences in gene expression profiles between SD and MF treatments for cells treated in vitro, with MF yielding a more robust induction compared with SD. Several genes were uniquely up-regulated by MF treatment, including multiple IFN-related genes (STAT1, G1P2, OAS1, 0AS3, G1P3, IFITM1) and TGF-β-associated genes (EGB1, VEGF, THBS1, and TGFB2). DU145 cells grown in vivo exhibited a completely different set of genes induced by both SD and MF compared with the same cells exposed in vitro. The results of the study clearly show distinct differences in the molecular response of cells between SD and MF radiation exposures and show that the tumor microenvironment can significantly influence the pattern of gene expression after radiation exposures. ?2007 American Association for Cancer Research. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-34248545443&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/332816 |
ISSN: | 00085472 | DOI: | 10.1158/0008-5472.CAN-06-4250 | SDG/關鍵字: | complementary DNA; messenger RNA; STAT1 protein; vasculotropin; article; breast adenocarcinoma; cancer survival; cell cycle; cell differentiation; gene control; gene expression; glioma cell; human; human cell; in vitro study; in vivo study; microarray analysis; priority journal; prostate carcinoma; radiation dose; radiation exposure; RNA isolation; solid tumor; tumor growth; upregulation; Animals; Breast Neoplasms; Cell Cycle; Cell Line, Tumor; Cluster Analysis; Dose Fractionation; Female; Gene Expression; Gene Expression Profiling; Genes, p53; Glioma; Humans; Interferons; Male; Mice; Mice, Nude; Neoplasm Transplantation; Oligonucleotide Array Sequence Analysis; Prostatic Neoplasms; Transforming Growth Factor beta; Transplantation, Heterologous; Up-Regulation |
顯示於: | 生物科技研究所 |
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