https://scholars.lib.ntu.edu.tw/handle/123456789/338223
Title: | Determination of camptothecins in DMSO extracts of Nothapodytes foetida by direct injection capillary electrophoresis | Authors: | Hsiao, H.-Y. Cheng, Tzong-Jih Yang, G.-M. Huang, I.-J. RICHIE CHEN |
Keywords: | 9-methoxycamptothecin; Hydrophobic compounds; MEKC; Organic modifier; Sample pretreatment | Issue Date: | 2008 | Journal Volume: | 19 | Journal Issue: | 2 | Start page/Pages: | 136-140 | Source: | Phytochemical Analysis | Abstract: | A rapid capillary electrophoresis procedure was developed for determining the anti-cancer components, camptothecins, in Nothapodytes foetida. The hydrophobic compound was extracted from plant tissue (ca. 1 mL of DMSO for 100mg of dried plant tissue) with a water-miscible organic solvent, DMSO, at elevated temperature (60°C). The extract was directly injected into the separation capillary (untreated fused silica, 34 cm in length, 75 μ i.d.) and analysed in MEKC mode (369 nm). Within 5 min of migration, camptothecins were successfully separated and quantified by adding organic modifiers to the running buffer (20% DMSO, 90 mM SDS in 10 mM borate buffer, pH 8.60). The linear dynamic range for camptothecin was from 5 to 400 μ/mL. This method was proven to be very suitable for monitoring the amount of camptothecins during the cultivation of the medicinal plant. Copyright ? 2007 John Wiley & Sons, Ltd. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-41149163216&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/338223 |
DOI: | 10.1002/pca.1026 | SDG/Keyword: | antineoplastic agent; boric acid; camptothecin; dimethyl sulfoxide; dodecyl sulfate sodium; Nothapodytes foetida extract; plant extract; unclassified drug; article; capillary electrophoresis; capillary zone electrophoresis; controlled study; drug determination; drug isolation; drug solubility; drug structure; hydrophobicity; linear system; medicinal plant; micellar electrokinetic chromatography; nonhuman; Nothapodytes foetida; pH measurement; quantitative analysis; temperature measurement; ultraviolet spectrophotometry; Angiosperms; Camptothecin; Dimethyl Sulfoxide; Electrophoresis, Capillary; Methanol; Molecular Structure; Plant Extracts; Solubility; Spectrophotometry, Ultraviolet; Nothapodytes foetida |
Appears in Collections: | 生物機電工程學系 |
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