https://scholars.lib.ntu.edu.tw/handle/123456789/347405
標題: | Modulation of morphology and functions of human hepatoblastoma cells by nano-grooved substrata | 作者: | Tsai, W.-B. Lin, J.-H. Tsai, WB Lin, JH WEI-BOR TSAI |
關鍵字: | Albumin secretion; Alignment; Hepatocyte; Nano-grooves/ridges; Urea | 公開日期: | 2009 | 卷: | 5 | 期: | 5 | 起(迄)頁: | 1442-1454 | 來源出版物: | Acta Biomaterialia | 摘要: | It is known that cellular behavior is affected by nano-patterned topography. For example, many cell types tend to align and extend along the direction of nano-grooves/ridges structures. In this study, we investigated the impact of nano-grooves/ridges on hepatocyte morphology and functions. HepG2/C3A (C3A) cells were cultured on nano-grooved silicon or polystyrene substrata with various widths (from 100 to 500 nm) and depths (from 100 to 380 nm). Nano-grooved substrates induced dramatic changes in C3A cell morphology. The cells formed spheroids on the flat substrates, while C3A cells spread and grew confluently with elongated and aligned morphology along the nano-grooves/ridges. Albumin synthesis was enhanced on the nano-grooved silicon substrates compared to the flat surface, and was decreased with increasing groove depths. Urea conversion on the shallow grooves (400 nm wide and 100 nm deep) remained at the same level of that on the flat surfaces, but was decreased on the deeper grooves. We found that the functions of hepatocytes were enhanced on the substrates with shallow grooves. The nano-grooved substrates may be applied as in vitro culture systems of hepatocytes for both diagnostic and therapeutic applications. ? 2009 Acta Materialia Inc. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-67349238313&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/347405 |
DOI: | 10.1016/j.actbio.2009.01.002 | SDG/關鍵字: | Cells; Cytology; Metabolism; Morphology; Substrates; Albumin secretion; Cell types; Cell-be; Cell/B.E; Cellular behaviors; Flatter surfaces; Hepatocytes; HepG2/C3A; Nano-groove/ridge; Ridge structures; Urea; albumin; silicon; urea; article; cancer cell culture; cell function; cell growth; cell spreading; cell strain HepG2; cell structure; controlled study; hepatoblastoma; human; human cell; in vitro study; liver cell; modulation; priority journal; protein synthesis; spheroid cell |
顯示於: | 化學工程學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。