https://scholars.lib.ntu.edu.tw/handle/123456789/347566
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | MIN-HSIUNG PAN | en_US |
dc.contributor.author | Lin, J.-H. | en_US |
dc.contributor.author | Lin-Shiau, S.-Y. | en_US |
dc.contributor.author | Lin, J.-K. | en_US |
dc.creator | Pan, M.-H.; Lin, J.-H.; Lin-Shiau, S.-Y.; Lin, J.-K. | - |
dc.date.accessioned | 2018-09-10T07:28:45Z | - |
dc.date.available | 2018-09-10T07:28:45Z | - |
dc.date.issued | 1999 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-0032748332&partnerID=MN8TOARS | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/347566 | - |
dc.description.abstract | Penta-O-galloyl-β-D-glucose is structurally related to (-)- epigallocatechin gallate and is isolated from hydrolyzed tannin. Penta-O- galloyl-β-D-glucose can inhibit tumor promotion by teleocidin. We investigated the effects of penta-O-galloyl-β-D-glucose and various tea polyphenols on cell viability in human leukemia HL-60 cells. In this study, we demonstrated that penta-O-galloyl-β-D-glucose was able to induce apoptosis in a concentration- and time-dependent manner; however, other polyphenols were less effective. We further investigated the molecular mechanisms of penta-O-galloyl-β-D-glucose-induced apoptosis. Treatment with penta-O-galloyl-β-D-glucose caused induction of caspase-3/CPP32 activity in dose- and time-dependent manner, but not caspase-1 activity, and induced the degradation of poly-(ADP-ribose) polymerase. Pretreatment with acetyl-Asp- Glu-Val-Asp-aldehyde (Ac-DEVD-CHO) and Z-Val-Ala-Asp-fluoromethylketone (Z- VAD-FMK) inhibited penta-O-galloyl-β-D-glucose-induced DNA fragmentation. Furthermore, treatment with penta-O-galloyl-β-D-glucose (50 μM) caused a rapid loss of mitochondrial transmembrane potential, release of mitochondrial cytochrome c into cytosol, and subsequent induction of procaspase-9 processing. Our results indicate that penta-O-galloyl-β-D-glucose allows caspase-activated deoxyribonuclease to enter the nucleus and degrade chromosomal DNA, and induces DFF-45 (DNA fragmentation factor) degradation. These results lead to a working hypothesis that penta-O-galloyl-β-D-glucose- induced apoptosis is triggered by the release of cytochrome c into the cytosol, procaspase-9 processing, activation of caspase-3, degradation of poly-(ADP-ribose) polymerase, and DNA fragmentation caused by the caspase- activated deoxyribonuclease through the digestion of DFF-45. The induction of apoptosis by penta-O-galloyl-β-D-glucose may provide a pivotal mechanism for its cancer chemopreventive action. | - |
dc.language | en | en |
dc.relation.ispartof | European Journal of Pharmacology | en_US |
dc.source | AH | - |
dc.subject | Apoptosis; Caspase- 3; Caspase-9; Caspase-activated deoxyribonuclease; Cytochrome c; DNA fragmentation factor; Penta-O-galloyl-β-D-glucose; Poly-(ADP-ribose) polymerase | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | caspase 3; DNA fragment; glucose pentagallate; interleukin 1beta converting enzyme; apoptosis; article; cancer prevention; cell viability; concentration response; DNA degradation; drug activity; enzyme activation; human; human cell; leukemia; priority journal; Acridine Orange; Apoptosis; Blotting, Western; Caspase 3; Caspases; Cell Division; DNA Fragmentation; DNA, Neoplasm; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Enzyme Inhibitors; Flow Cytometry; Fluorescent Dyes; HL-60 Cells; Humans; Hydrolyzable Tannins; Membrane Potentials; Mitochondria; Na(+)-K(+)-Exchanging ATPase; Subcellular Fractions; Tannins | - |
dc.title | Induction of apoptosis by penta-O-galloyl-β-D-glucose through activation of caspase-3 in human leukemia HL-60 cells | - |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/S0014-2999(99)00549-X | - |
dc.relation.pages | 171-183 | - |
dc.relation.journalvolume | 381 | - |
dc.relation.journalissue | 2-3 | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
crisitem.author.dept | Food Science and Technology | - |
crisitem.author.orcid | 0000-0002-5188-7030 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 食品科技研究所 |
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