https://scholars.lib.ntu.edu.tw/handle/123456789/347728
Title: | Size effect on cell uptake in well-suspended, uniform mesoporous silica nanoparticles | Authors: | Lu, F. Wu, S.-H. Hung, Y. CHUNG-YUAN MOU |
Keywords: | Cell uptake; Mesoporous materials; Nanoparticles; Particle size; Silica | Issue Date: | 2009 | Journal Volume: | 5 | Journal Issue: | 12 | Start page/Pages: | 1408-1413 | Source: | Small | Abstract: | A study was conducted to demonstrate the synthesis of monodisperse mesoporous silica nanoparticle (MSN) suspensions and investigate the effect of nanoparticle size on cell uptake. The MSNs were synthesized by adjusting the pH of the reaction medium, leading to a series of MSNs with uniform size ranging from 30-280 nm. The physical properties of the MSNs were characterized with various methods, such as nitrogen adsorption-desorption, transmission electron microscopy (TEM), and X-ray powder diffraction (XRD). The particle size effect on the cell uptake efficiency with human cervical cancer cells and cellular uptake of MSNs of various sizes were investigated by confocal laser scanning microscopy (CLSM). The uptake efficiencies were determined by inductively coupled plasma mass spectrometry (ICP-MS) analysis of total silicon content. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-67650338048&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/347728 |
DOI: | 10.1002/smll.200900005 | SDG/Keyword: | Cell uptake; Cellular uptake; Cervical cancer cells; Confocal laser scanning microscopy; Mesoporous silica nanoparticles; Monodisperse; Nanoparticle sizes; Nitrogen adsorption-desorption; Particle size effect; Reaction medium; Silicon contents; Size effects; TEM; Uniform size; XRD; Cell membranes; Confocal microscopy; Desorption; Gas adsorption; Inductively coupled plasma; Inductively coupled plasma mass spectrometry; Mass spectrometers; Nanoparticles; Particle size; Silica; Synthesis (chemical); Transmission electron microscopy; Mesoporous materials; fluorescein isothiocyanate; nanoparticle; silicon dioxide; article; chemistry; confocal microscopy; HeLa cell; human; light; particle size; pH; porosity; radiation scattering; transmission electron microscopy; ultrastructure; X ray diffraction; Fluorescein-5-isothiocyanate; Hela Cells; Humans; Hydrogen-Ion Concentration; Light; Microscopy, Confocal; Microscopy, Electron, Transmission; Nanoparticles; Particle Size; Porosity; Scattering, Radiation; Silicon Dioxide; X-Ray Diffraction |
Appears in Collections: | 化學系 |
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