https://scholars.lib.ntu.edu.tw/handle/123456789/355628
標題: | Micro-sandwich in microfluidics: 3D biopolymer membranes for cell assembly | 作者: | Luo, X.L. Wu, H.C. Tsao, C.Y. Cheng, Y. Rubloff, G.W. Bentley, W.E. HSUAN-CHEN WU |
關鍵字: | Biofabrication; Cell assembly; Membrane; Three dimensional | 公開日期: | 2010 | 卷: | 3 | 起(迄)頁: | 1601-1603 | 來源出版物: | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences | 摘要: | We demonstrate the biofabrication of three dimensional (3D) biopolymer membranes and the assembly of two cell lines in a micro-sandwich structure in microfluidics. A freestanding chitosan membrane was first fabricated by using pH gradients generated at the flow interface of two converging flows. The micro-sandwich was then fabricated by cross-linking alginate on both sides of the chitosan membrane with diffusion of calcium ions through the semi-permeable chitosan membrane. Cell assembly was achieved by blending cells into the alginate solution to embed the target cells into the micro-sandwich alginate scaffolds. The cell assembly process is simple, fast and easy to control. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84884321397&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/355628 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84884321397&partnerID=40&md5=a1b96477c6dbb7050cf5ca25093297ef |
SDG/關鍵字: | Alginate scaffolds; Alginate solutions; Biofabrication; Cell assembly; Chitosan membrane; Converging flow; Flow interface; Target cells; Alginate; Biomolecules; Blending; Cell culture; Cells; Chitosan; Membranes; Microfluidics; Three dimensional; Scaffolds (biology) |
顯示於: | 生化科技學系 |
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