https://scholars.lib.ntu.edu.tw/handle/123456789/356137
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Chia, M.-Y. | en_US |
dc.contributor.author | Hsiao, S.-H. | en_US |
dc.contributor.author | Chan, H.-T. | en_US |
dc.contributor.author | YI-YIN DO | en_US |
dc.contributor.author | Huang, P.-L. | en_US |
dc.contributor.author | HUI-WEN CHANG | en_US |
dc.contributor.author | Tsai, Y.-C. | en_US |
dc.contributor.author | Lin, C.-M. | en_US |
dc.contributor.author | VICTOR FEI PANG | en_US |
dc.contributor.author | CHIAN-REN JENG | en_US |
dc.creator | Chia, M.-Y.;Hsiao, S.-H.;Chan, H.-T.;Do, Y.-Y.;Huang, P.-L.;Chang, H.-W.;Tsai, Y.-C.;Lin, C.-M.;Pang, V.F.;Jeng, C.-R. | - |
dc.date.accessioned | 2018-09-10T08:09:02Z | - |
dc.date.available | 2018-09-10T08:09:02Z | - |
dc.date.issued | 2010 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-77952548054&partnerID=MN8TOARS | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/356137 | - |
dc.description.abstract | The aim of the study was to evaluate the immunogenicity of the ORF5-encoded major envelop glycoprotein 5 (GP5) of porcine reproductive and respiratory syndrome virus (PRRSV) expressed in tobacco plant as a potential pig oral vaccine in protection against PRRSV infection. Six-week-old PRRSV-free pigs were fed four times orally with 50. g of chopped fresh GP5 transgenic tobacco leaves (GP5-T) (GP5 reaching 0.011% of total soluble protein) or wild-type tobacco leaves (W-T) each on days 0, 14, 28, and 42. Samples of serum, saliva, and peripheral blood mononuclear cells (PBMCs) were collected on days -1, 6, 13, 20, 27, 34, 41, and 48 after the initial oral vaccination. A similar vaccination-dependent gradual increase in the responses of serum and saliva anti-PRRSV total IgG and IgA, respectively, and in the levels of PRRSV-specific blastogenic response of PBMCs was seen in GP5-T-treated pigs; all statistically significant elevations occurred after the 2nd vaccination and were revealed after 20 days post-initial oral vaccination (DPIOV). Pigs fed on GP5-T also developed serum neutralizing antibodies to PRRSV at a titer of 1:4-1:8 after the 4th vaccination by 48 DPIOV. No detectable anti-PRRSV antibody responses and PRRSV-specific blastogenic response were seen in W-T-treated pigs. The present study has demonstrated that pigs fed on GP5-T could develop specific mucosal as well as systemic humoral and cellular immune responses against PRRSV. The results also support that transgenic plant as GP5-T can be an effective system for oral delivery of recombinant subunit vaccines in pigs. ? 2009 Elsevier B.V. | - |
dc.format | application/pdf | en |
dc.format.extent | 273682 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | en | en |
dc.relation | Veterinary Immunology and Immunopathology 135 (3-4): 234-242 | en |
dc.relation.ispartof | Veterinary Immunology and Immunopathology | en_US |
dc.source | AH | - |
dc.subject | Glycoprotein 5; PRRSV; Transgenic tobacco leaves | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | edible vaccine; immunoglobulin A antibody; immunoglobulin G antibody; neutralizing antibody; porcine reproductive and respiratory syndrome vaccine; recombinant glycoprotein gp 5; recombinant protein; unclassified drug; virus vaccine; animal experiment; antibody blood level; antibody response; antibody titer; article; cellular immunity; controlled study; humoral immunity; immunogenicity; lymphocyte proliferation; male; mucosal immunity; nonhuman; nucleotide sequence; open reading frame; peripheral blood mononuclear cell; porcine reproductive and respiratory syndrome; saliva analysis; tobacco; transgenic plant; virus neutralization; Administration, Oral; Animals; Antibodies, Neutralizing; Antibodies, Viral; Base Sequence; Bioreactors; DNA, Viral; Immunity, Cellular; Immunity, Humoral; Immunity, Mucosal; Immunoglobulin A, Secretory; Immunoglobulin G; Lymphocyte Activation; Male; Plants, Genetically Modified; Porcine Reproductive and Respiratory Syndrome; Porcine respiratory and reproductive syndrome virus; Saliva; Sus scrofa; Swine; Tobacco; Vaccines, Edible; Viral Envelope Proteins; Viral Vaccines; Nicotiana obtusifolia; Nicotiana tabacum; Porcine respiratory and reproductive syndrome virus; Suidae | - |
dc.title | Immunogenicity of recombinant GP5 protein of porcine reproductive and respiratory syndrome virus expressed in tobacco plant | - |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.vetimm.2009.12.003 | - |
dc.relation.pages | 234-242 | - |
dc.relation.journalvolume | 135 | - |
dc.relation.journalissue | 3-4 | - |
item.openairetype | journal article | - |
item.fulltext | with fulltext | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | open | - |
crisitem.author.dept | Horticulture and Landscape Architecture | - |
crisitem.author.dept | Master Program in Global Agriculture Technology and Genomic Science (Global ATGS) | - |
crisitem.author.dept | Molecular and Comparative Pathobiology | - |
crisitem.author.dept | Veterinary Medicine | - |
crisitem.author.dept | Molecular and Comparative Pathobiology | - |
crisitem.author.orcid | 0000-0002-2142-2486 | - |
crisitem.author.orcid | 0000-0001-8877-1886 | - |
crisitem.author.orcid | 0000-0002-8048-4548 | - |
crisitem.author.orcid | 0000-0003-3941-6276 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | International College | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 園藝暨景觀學系 |
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