https://scholars.lib.ntu.edu.tw/handle/123456789/356301
Title: | Lucidone, a novel melanin inhibitor from the fruit of Lindera erythrocarpa Makino | Authors: | Kumar, K. J. S. Yang, J. C. Chu, F. H. Chang, S. T. Wang, S. Y. SHANG-TZEN CHANG FANG-HUA CHU |
Keywords: | Antimelanogenic; Lucidone; Melanin; MITF; Tyrosinase inhibition | Issue Date: | 2010 | Journal Volume: | 24 | Journal Issue: | 8 | Start page/Pages: | 1158-1165 | Source: | Phytotherapy Research | Abstract: | The effects of lucidone on tyrosinase and antimelanogenic activity were investigated. Initially, we found that lucidone strongly inhibits the activity of mushroom tyrosinase. The effects of lucidone on tyrosinase were further examined in α-MSH-induced B16 melanoma cells. Lucidone significantly inhibits tyrosinase activity and leads to decreased melanin content in cultured B16 melanoma cells. Lucidone also attenuates the expression of tyrosinase and MITF (Microphthalmia-associated Transcription Factor) protein in a concentration-dependent manner, as shown by western blot. Quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) confirmed that lucidone inhibits the expression of tyrosinase mRNA. Accordingly, the effects of lucidone on the ERK signaling pathway were also investigated, but lucidone was not found to play major role in the induction of ERK activation. Our data indicate that the antimelanogenic activity of lucidone is probably due to its inhibition of tyrosinase activity and the suppression of tyrosinase and MITF expression. Copyright ? 2010 John Wiley & Sons, Ltd. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-77955595628&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/356301 |
DOI: | 10.1002/ptr.3018 | SDG/Keyword: | lucidone; melanin; microphthalmia associated transcription factor; mitogen activated protein kinase; monophenol monooxygenase; plant extract; unclassified drug; cyclopentane derivative; lucidone; melanin; messenger RNA; microphthalmia associated transcription factor; monophenol monooxygenase; animal cell; article; cancer cell culture; concentration response; controlled study; drug effect; enzyme inhibition; fruit; Lindera; Lindera erythrocarpa; melanogenesis; melanoma B16; mouse; nonhuman; protein expression; Agaricales; animal; antagonists and inhibitors; biosynthesis; chemistry; enzymology; experimental melanoma; gene expression regulation; metabolism; reverse transcription polymerase chain reaction; Basidiomycota; Lindera erythrocarpa; Agaricales; Animals; Cyclopentanes; Gene Expression Regulation; Lindera; Melanins; Melanoma, Experimental; Mice; Microphthalmia-Associated Transcription Factor; Monophenol Monooxygenase; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger |
Appears in Collections: | 森林環境暨資源學系 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.