|Title:||A type-specific blocking ELISA for the detection of infectious bronchitis virus antibody||Authors:||Chen, H.-W.
|Keywords:||Blocking ELISA; Hemagglutination inhibition test; Infectious bronchitis virus; Monoclonal antibody||Issue Date:||2011||Journal Volume:||173||Journal Issue:||1||Start page/Pages:||7-12||Source:||Journal of Virological Methods||Abstract:||
Infectious bronchitis virus (IBV) infection has been a major threat to the poultry industry worldwide. Current commercially available ELISA kits detect group-specific antibodies; however, to understand the status of field infection, a monoclonal antibody (mAb) blocking ELISA (b-ELISA) against local IBVs was developed. The selected mAb showed specificity to Taiwan IBV strains but had no cross-reactivity with the vaccine strain H120. Using the hemagglutination inhibition (HI) test as a standard, the cut-off value, sensitivity, and specificity of a b-ELISA using this mAb were evaluated in 390 field samples. The type-specificity of detection was validated using a panel of chicken hyperimmune sera. The results showed that the b-ELISA demonstrated high sensitivity (98.0%) and specificity (97.2%) of detection. The agreement between the results of the b-ELISA and the HI test was statistically significant (Kappa. =0.95), and there was no significant difference between these two methods (McNemar p=0.72). The b-ELISA specifically detected Taiwan IBV serotypes but not three non-Taiwan IBV serotypes nor sera against other avian pathogens. This b-ELISA provides type-specific antibody detection of local IBV strains. It has the potential to serve as a rapid and reliable diagnostic method for IBV clinical infections in the field in Taiwan. ? 2011 Elsevier B.V.
|DOI:||10.1016/j.jviromet.2010.12.016||metadata.dc.subject.other:||monoclonal antibody; monoclonal antibody QI3 4; unclassified drug; animal cell; antibody detection; antibody specificity; article; Avian infectious bronchitis virus; blocking enzyme linked immunosorbent assay; controlled study; enzyme linked immunosorbent assay; hemagglutination inhibition test; intermethod comparison; nonhuman; priority journal; reference value; sensitivity and specificity; serotype; Taiwan; virus strain; Animals; Antibodies, Monoclonal; Antibodies, Viral; Chickens; Coronavirus Infections; Enzyme-Linked Immunosorbent Assay; Infectious bronchitis virus; Poultry Diseases; Sensitivity and Specificity; Taiwan; Virology; Aves; Avian infectious bronchitis virus
|Appears in Collections:||獸醫學系|
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