https://scholars.lib.ntu.edu.tw/handle/123456789/362357
標題: | Cellular photodynamic toxicity of hematoporphyrin in various nanocarrier systems | 作者: | Yang, Y.-T. Chen, C.-T. Chien, H.-F. Tsai, T. CHIN-TIN CHEN |
關鍵字: | Liposomes; Micelles; Nanoparticles; Photodynamic therapy; Photosensitizers; Protein binding | 公開日期: | 2011 | 卷: | 7 | 期: | 6 | 起(迄)頁: | 850-855 | 來源出版物: | Current Nanoscience | 摘要: | In this study, we investigated the cellular uptake of Hematoporphyrin (Hp) encapsulated in nanocarriers by human lung adenocarcinoma A549 cells and the photodynamic toxic effects on those cells. The nanocarriers included liposomes, micelles, and nanoparticles. The Hp was encapsulated in liposomes through a film hydration and extrusion method. Hp was encapsulated in micelles through a film hydration method. The Hp loaded nanoparticles were prepared using an emulsification-diffusion process. The Hp in nanocarriers was characterized by laser light scattering to determine particle size and zeta potential of the surface charge. The cellular uptake of nanocarriers was measured by fluorescence spectrometry. The photodynamic toxicity of various Hp nanocarriers was evaluated in human lung epithelial carcinoma A549 cells using a light-emitting diode (LED) device at a wavelength of 630±5 nm. The averaged size of all nanocarriers was between 112 and 135 nm. The surface charge observed for all formulations, particularly the micelles showed lower negative valued than those of other systems. Cellular uptake and photocytotoxicity of the four Hp preparations in serum free medium were in the following order: micelle-Hp > nanoparticle-Hp > liposome-Hp > free Hp. In the presence of serum protein, the photodynamic toxicity of all formulations significantly decreased. ? 2011 Bentham Science Publishers. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-82155170749&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/362357 |
DOI: | 10.2174/157341311798220718 | SDG/關鍵字: | A549 cells; Cellular uptake; Extrusion method; Fluorescence spectrometry; HematoPorphyrin; Human lung; Hydration method; Laser light scattering; Nanocarriers; Photocytotoxicity; Protein binding; Serum proteins; Serum-free medium; Toxic effect; Biochemistry; Biological organs; Body fluids; Cells; Emulsification; Film preparation; Fluorescence spectroscopy; Hydration; Light scattering; Liposomes; Micelles; Nanoparticles; Phospholipids; Photodynamic therapy; Photosensitizers; Porphyrins; Respiratory system; Toxicity; Zeta potential; Light emitting diodes; hematoporphyrin; liposome; nanocarrier; plasma protein; antineoplastic activity; article; cancer cell culture; carcinoma cell; cell transport; controlled study; culture medium; diffusion; drug cytotoxicity; drug delivery system; drug release; drug uptake; emulsion; human; human cell; hydration; light emitting diode; light scattering; liposomal delivery; lung adenocarcinoma; micelle; nanoencapsulation; particle size; phototoxicity; priority journal; slow drug release; spectrofluorometry; surface charge; zeta potential |
顯示於: | 生化科技學系 |
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