https://scholars.lib.ntu.edu.tw/handle/123456789/365575
標題: | Isolation and characterization of a Bacillus licheniformis strain capable of degrading zearalenone | 作者: | Yi, P.-J. Pai, C.-K. JE-RUEI LIU |
關鍵字: | Bacillus licheniformis; Biodegradation; Cellulase; Xylanase; Zearalenone | 公開日期: | 2011 | 卷: | 27 | 期: | 5 | 起(迄)頁: | 1035-1043 | 來源出版物: | World Journal of Microbiology and Biotechnology | 摘要: | The worldwide contamination of cereals, oilseeds, and other crops by mycotoxin-producing moulds is a significant problem. Mycotoxins have adverse effects on humans and animals that result in illnesses and economic losses. Reduction or elimination of mycotoxin contamination in food and feed is an important issue. This study aimed to screen soil bacteria for degradation of zearalenone (ZEN). A pure culture of strain CK1 isolated from soil samples showed most capable of degradation of ZEN. Using physiological, biochemical, and 16S rRNA gene sequence analysis methods, CK1 was identified as Bacillus licheniformis. Addition of 2 ppm of ZEN in Luria-Bertani (LB) medium, B. licheniformis CK1 decreased 95. 8% of ZEN after 36 h of incubation. In ZEN-contaminated corn meal medium, B. licheniformis CK1 decreased more than 98% of ZEN after 36 h of incubation. In addition, B. licheniformis CK1 was non-hemolytic, non-enterotoxin producing, and displayed high levels of extracellular xylanase, cellulase, and protease activities. These findings suggest that B. licheniformis CK1 could be used to reduce the concentrations of ZEN and improve the digestibility of nutrients in feedstuffs simultaneously. ? 2010 Springer Science+Business Media B.V. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-79955051768&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/365575 |
DOI: | 10.1007/s11274-010-0548-7 | SDG/關鍵字: | 16S rRNA gene sequence; Adverse effect; Bacillus licheniformis; Cellulase; Corn meal; Economic loss; Enterotoxin; Extracellular; Feedstuffs; Isolation and characterization; Luria-bertani mediums; Protease activities; Pure culture; Soil bacterium; Soil sample; Xylanases; Zearalenone; Animals; Bacilli; Bacteriology; Biodegradation; Degradation; Genes; Losses; RNA; Chemical contamination; Animalia; Bacillus licheniformis; Luria; Zea mays |
顯示於: | 生物科技研究所 |
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