https://scholars.lib.ntu.edu.tw/handle/123456789/367842
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | CHUN-JUNG LIN | en |
dc.creator | Yeh, S.-Y.;Pan, H.-J.;Lin, C.-C.;Kao, Y.-H.;Chen, Y.-H.;Lin, C.-J. | - |
dc.date.accessioned | 2018-09-10T09:13:01Z | - |
dc.date.available | 2018-09-10T09:13:01Z | - |
dc.date.issued | 2012 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-84864468778&partnerID=MN8TOARS | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/367842 | - |
dc.description.abstract | The purpose of this study is to investigate the regulation of P-glycoprotein expression in the kidney under diabetic condition. Renal P-glycoprotein expression was examined in inbred mice with type 1 or type 2 diabetes by Western blotting. The underlying mechanisms of P-glycoprotein regulation were examined in Madin-Darby canine kidney type II (MDCK-II) cells by Western blotting or qRT-PCR. 3H-digoxin uptake was measured for P-glycoprotein activity in cells under various treatments. The results showed that P-glycoprotein expression was lower in kidneys of diabetic mice than in controls. In MDCK-II cells, treatments with insulin or IL-6 did not cause any change in P-glycoprotein expression, whereas TNF-α tended to increase P-glycoprotein expression at a concentration of 1 ng/ml. On the other hand, P-glycoprotein expression was reduced under high glucose conditions (450 mg/dl), while superoxide production was increased, and the reduction in P-glycoprotein expression was abolished by N-acetylcysteine (an antioxidant) and staurosporine (a nonselective PKC inhibitor). Treatment with oxidizing agents (H 2O2, BSO) or PMA (a PKC activator) reduced P-glycoprotein expression. Antioxidant (N-acetylcysteine or glutathione) co-treatment abolished the H2O2-induced and BSO-induced reduction in P-glycoprotein expression, whereas it did not prevent the effect of PMA. The PMA-induced P-glycoprotein down-regulation was prevented by co-treatment of LY333531 (a PKC-β inhibitor). 3H-digoxin levels were higher in MDCK-II cells with high glucose, PMA or H2O2 treatments. In conclusion, P-glycoprotein expression is lower in kidneys of diabetic mice and in MDCK-II cells under high glucose conditions. Hyperglycemia induced reactive oxygen species and activated PKC in MDCK-II cells, leading to the decrease in P-glycoprotein expression. ? 2012 Elsevier B.V. | - |
dc.language | en | en |
dc.relation.ispartof | European Journal of Pharmacology | en_US |
dc.source | AH | - |
dc.subject | Diabetes; Kidney; P-glycoprotein; PKC; Reactive oxygen species | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | acetylcysteine; digoxin; digoxin h 3; glucose; glutathione; glycoprotein P; insulin; interleukin 6; oxidizing agent; reactive oxygen metabolite; ruboxistaurin; staurosporine; superoxide; tumor necrosis factor alpha; unclassified drug; animal cell; animal experiment; article; concentration response; controlled study; down regulation; female; hyperglycemia; insulin dependent diabetes mellitus; kidney cell; male; mouse; non insulin dependent diabetes mellitus; nonhuman; priority journal; protein expression; regulatory mechanism; Animals; Biological Transport; Blood Glucose; Body Weight; Cell Membrane; Diabetes Complications; Diabetes Mellitus, Type 1; Diabetes Mellitus, Type 2; Digoxin; Dogs; Down-Regulation; Female; Hyperglycemia; Insulin; Interleukin-6; Kidney; Madin Darby Canine Kidney Cells; Male; Mice; P-Glycoprotein; Protein Kinase C; Reactive Oxygen Species; Tumor Necrosis Factor-alpha | - |
dc.title | Hyperglycemia induced down-regulation of renal P-glycoprotein expression | - |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.ejphar.2012.06.013 | - |
dc.relation.pages | 42-50 | - |
dc.relation.journalvolume | 690 | - |
dc.relation.journalissue | 1-3 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Pharmacy | - |
crisitem.author.dept | School of Pharmacy | - |
crisitem.author.orcid | 0000-0001-9220-3507 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 藥學系 |
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