https://scholars.lib.ntu.edu.tw/handle/123456789/369529
標題: | The role of porcine teschovirus in causing diseases in endemically infected pigs | 作者: | Chiu, Shu-Chun Hu, Shu-Chia Chang, Chih-Cheng CHIA-YI CHANG Huang, Chin-Cheng VICTOR FEI PANG FUN-IN WANG |
公開日期: | 2012 | 卷: | 161 | 期: | 1-2 | 起(迄)頁: | 88-95 | 來源出版物: | Veterinary Microbiology | 摘要: | Porcine teschoviruses (PTVs) belong to the genus Teschovirus within the family Picornaviridae. Hitherto, PTVs have had 13 serotypes associated with a variety of clinical diseases. The virulent PTV-1 strains were associated with highly fatal, nonsuppurative encephalomyelitis of pigs (Teschen disease) in the 1930-1950s. Today, less virulent Talfan strains of PTV-1 are more widespread, and PTVs have contaminated swine herds worldwide (endemic or enzootic) together with a variety of common swine pathogens (multi-infection status). The aim of this study was to investigate the extent to which PTVs play a role in causing diseases in the field, under the endemic and multi-infection situation, when most pigs in the herds are infected and immune. Based on the fecal-oral model of pathogenesis, a set of 15 organs were collected from 30 culled post-weanling piglets of 4-8 weeks old. For nested RT-PCR targeted on the 5'-NTR, the PTV detection rate was 96.7% (by heads), confirming the endemic status, and infection was most commonly detected in the intestines (averaged 61%) and lymphoid organs (averaged 59%), followed by visceral organs (averaged 37%) and the CNS (different parts varied from 17 to 47%). The correlation of PTVs detected by nested RT-PCR and a histological lesion were analyzed by Chi-square test showing that in the field situation only non-suppurative encephalitis in the caudal part of the brain (P= 0.054) may be marginal significantly attributed to infection by PTVs. By genotyping based on partial VP1 sequences, 5 serotypes, namely PTV-1, -4, -6, -7, and -11, were identified, with some animals having two serotypes co-existed in different organs. © 2012 Elsevier B.V. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84869864594&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/369529 |
DOI: | 10.1016/j.vetmic.2012.07.031 | SDG/關鍵字: | Endemic; Multi-infection; Nonsuppurative encephalitis; Porcine teschovirus Animalia; Peru tomato mosaic virus; Picornaviridae; Porcine teschovirus; Suidae; Sus; Teschovirus animal cell; animal experiment; animal tissue; article; brain; central nervous system; controlled study; encephalitis; gene sequence; genotype; histopathology; intestine; lymph node; nonhuman; reverse transcription polymerase chain reaction; serotype; swine; Teschen disease; Teschovirus; virus detection; viscera protein VP1 |
顯示於: | 分子暨比較病理生物學研究所 |
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