https://scholars.lib.ntu.edu.tw/handle/123456789/378020
標題: | Middle infrared radiation induces G2/M cell cycle arrest in A549 lung cancer cells | 作者: | HSUEH-FEN JUAN SI-CHEN LEE |
公開日期: | 2013 | 出版社: | PLOS | 卷: | 8 | 期: | 1 | 起(迄)頁: | e54117 | 來源出版物: | PLoS ONE | 摘要: | There were studies investigating the effects of broadband infrared radiation (IR) on cancer cell, while the influences of middle-infrared radiation (MIR) are still unknown. In this study, a MIR emitter with emission wavelength band in the 3-5 μm region was developed to irradiate A549 lung adenocarcinoma cells. It was found that MIR exposure inhibited cell proliferation and induced morphological changes by altering the cellular distribution of cytoskeletal components. Using quantitative PCR, we found that MIR promoted the expression levels of ATM (ataxia telangiectasia mutated), ATR (ataxia-telangiectasia and Rad3-related and Rad3-related), TP53 (tumor protein p53), p21 (CDKN1A, cyclin-dependent kinase inhibitor 1A) and GADD45 (growth arrest and DNA-damage inducible), but decreased the expression levels of cyclin B coding genes, CCNB1 and CCNB2, as well as CDK1 (Cyclin-dependent kinase 1). The reduction of protein expression levels of CDC25C, cyclin B1 and the phosphorylation of CDK1 at Thr-161 altogether suggest G2/M arrest occurred in A549 cells by MIR. DNA repair foci formation of DNA double-strand breaks (DSB) marker γ-H2AX and sensor 53BP1 was induced by MIR treatment, it implies the MIR induced G2/M cell cycle arrest resulted from DSB. This study illustrates a potential role for the use of MIR in lung cancer therapy by initiating DSB and blocking cell cycle progression. ? 2013 Chang et al. |
URI: | http://europepmc.org/abstract/med/23335992 http://scholars.lib.ntu.edu.tw/handle/123456789/378020 |
DOI: | 10.1371/journal.pone.0054117 | SDG/關鍵字: | ATM protein; ATR protein; cyclin B; cyclin dependent kinase 1; cyclin dependent kinase inhibitor 1A; growth arrest and DNA damage inducible protein 45; histone H2AX; protein p21; protein p53; article; cancer cell culture; carcinoma cell; cell cycle arrest; cell cycle G2 phase; cell cycle M phase; cell cycle progression; cell proliferation; cell strain A549; cell structure; cell viability; cellular distribution; controlled study; cytoskeleton; DNA repair; double stranded DNA break; human; human cell; immunofluorescence; infrared radiation; lung adenocarcinoma; middle infrared radiation; protein expression; radiation exposure; real time polymerase chain reaction; reverse transcription; RNA extraction; Western blotting; Actins; CDC2 Protein Kinase; cdc25 Phosphatases; Cell Culture Techniques; Cell Cycle Proteins; Cell Line, Tumor; Cell Proliferation; Culture Media; Cyclin B1; DNA Damage; G2 Phase Cell Cycle Checkpoints; Gene Expression Regulation, Neoplastic; Histones; Humans; Infrared Rays; Intracellular Signaling Peptides and Proteins; Lung Neoplasms; M Phase Cell Cycle Checkpoints; Microtubules; Phosphorylation; Protein Binding; Protein Transport; Reactive Oxygen Species; RNA, Messenger; Temperature; Vinculin; Ataxia telangiectasia |
顯示於: | 生命科學系 |
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