https://scholars.lib.ntu.edu.tw/handle/123456789/378221
標題: | Phospholipid-functionalized mesoporous silica nanocarriers forselective photodynamic therapy of cancer | 作者: | Teng, I.-T. Chang, Y.-J. Wang, L.-S. Lu, H.-Y. Wu, L.-C. Yang, C.-M. Chiu, C.-C. Yang, C.-H. Hsu, S.-L. Ho, J.A.A. JA-AN ANNIE HO |
關鍵字: | Active targeting; Mesoporous silica nanoparticle; Phospholipid capping; Photocytotoxicity; Photodynamic cancer therapy | 公開日期: | 2013 | 卷: | 34 | 期: | 30 | 起(迄)頁: | 7462-7470 | 來源出版物: | Biomaterials | 摘要: | This paper describes the fabrication of a highly efficient, non-cytotoxic drug delivery platform designed for photodynamic therapy (PDT): phospholipid-capped, protoporphyrin IX-loaded and FITC-sensitized mesoporous silica nanocarriers (Lipo-FMSNs/PpIX). After derivatization with folate on the phospholipid-capped FMSNs (denoted fa-Lipo-FMSNs/PpIX, the so-called nanoPDT system), we confirmed the nanoPDT systems' selective targeting of and entry into the folic acid receptor-overexpressed HeLa cells by means of cell viability assessment and confocal microscopic analysis. The decrease in the unfavorable dark toxicity of fa-Lipo-FMSNs/PpIX enabled the delivery of high concentrations of PpIX into cells. Moreover, the cellular uptake of the nanoPDT systems was greater than that of free PpIX. Upon irradiation with visible light, the nanoPDT system generated singlet oxygen efficaciously in aqueous environments-a decisive factor affecting its therapeutic applicability in PDT, demonstrating enhanced invitro photocytotoxicity. Furthermore, an invivo study of subcutaneous melanoma in nude mice inoculated with B16F10 cells revealed the capability for the nanoPDT system to mitigate nearly 65% of tumor growth. ? 2013 Elsevier Ltd. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84880514407&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/378221 |
DOI: | 10.1016/j.biomaterials.2013.06.001 | SDG/關鍵字: | Active targeting; Cancer therapy; Mesoporous Silica; Mesoporous silica nanoparticles; Microscopic analysis; Photocytotoxicity; Photodynamic therapy (PDT); Protoporphyrins; Drug delivery; Oncology; Photodynamic therapy; Phospholipids; caspase 3; caspase 8; cyclin D; cyclin D1; cytochrome c; nanocarrier; phospholipid; protoporphyrin; retinoblastoma protein; silica nanocarrier; silicon dioxide; singlet oxygen; unclassified drug; animal experiment; apoptosis; article; cancer therapy; cell cycle progression; cell cycle S phase; cell damage; cell proliferation; cell survival; cell viability; concentration (parameters); controlled study; derivatization; encapsulation; HeLa cell; histogram; human; human cell; in vitro study; in vivo study; internalization; irradiation; mouse; nonhuman; photodynamic therapy; photolysis; phototoxicity; priority journal; tumor volume; ultraviolet irradiation; Active targeting; Mesoporous silica nanoparticle; Phospholipid capping; Photocytotoxicity; Photodynamic cancer therapy; Animals; Antineoplastic Agents; Cell Survival; Drug Carriers; Endocytosis; Fluorescence; Folic Acid; HeLa Cells; Humans; Immunohistochemistry; Intracellular Space; Male; Melanoma, Experimental; Mice; Mice, Nude; Nanoparticles; Neoplasms; Phospholipids; Photochemotherapy; Porosity; Protoporphyrins; Silicon Dioxide; Mus musculus |
顯示於: | 生化科技學系 |
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