https://scholars.lib.ntu.edu.tw/handle/123456789/380685
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Yang, Y.-C. | en_US |
dc.contributor.author | Yang-Chih Shih, D. | en_US |
dc.contributor.author | MONG-HSUN TSAI | en_US |
dc.contributor.author | Cheng, C.-H. | en_US |
dc.contributor.author | Cheng, H.-F. | en_US |
dc.contributor.author | Lo, C.-F. | en_US |
dc.contributor.author | Wang, D.-Y. | en_US |
dc.creator | Yang, Y.-C.;Yang-Chih Shih, D.;Tsai, M.-H.;Cheng, C.-H.;Cheng, H.-F.;Lo, C.-F.;Wang, D.-Y. | - |
dc.date.accessioned | 2018-09-10T09:49:08Z | - |
dc.date.available | 2018-09-10T09:49:08Z | - |
dc.date.issued | 2013 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-84878237417&partnerID=MN8TOARS | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/380685 | - |
dc.description.abstract | The World Health Organization (WHO) International Standard (IS) for human immunodeficiency virus type 1 (HIV-1) RNA is only available in limited amounts. It is critical to use the most common HIV-1 genotype as source for secondary standards, e.g. a National Standard for Taiwan. The objective of this study was to establish the first National Standard for HIV-1 RNA NAT assays in Taiwan. A collaborative study, including eleven laboratories from five different countries, was carried out to establish the HIV-1 RNA National Standard by calibration, in International Units (IU), against the WHO HIV-1 RNA IS. The HIV-1 RNA content for the candidate was quantitated by each laboratory in three independent assays and the results were collected and analyzed statistically. Overall, a high level of agreement among results was achieved from different laboratories. In addition, the stability study indicated that the candidate was stable for 24 months at -80 ± 5. °C. In conclusion, the candidate standard was established as the first National Standard for HIV-1 RNA for use in NAT assays in Taiwan. The standard is intended to be used for the quality control of HIV-1 NAT assays and as a quantitative reference material for HIV-1 NAT assays. ? 2013 Elsevier B.V. | - |
dc.language | en | en |
dc.relation.ispartof | Journal of Virological Methods | en_US |
dc.source | AH-Scopus to ORCID | - |
dc.subject | Human immunodeficiency virus type 1 (HIV-1); Nucleic acid amplification techniques (NAT); Standard | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | article; bioassay; clinical laboratory; clinical study; controlled study; genotype; health care quality; human; Human immunodeficiency virus 1; nonhuman; nucleic acid amplification; priority journal; quality control; quantitative analysis; reference value; standard; Taiwan; HIV Infections; HIV-1; Humans; International Cooperation; Nucleic Acid Amplification Techniques; Reference Standards; RNA, Viral; Taiwan; Viral Load | - |
dc.title | A collaborative study to establish the first National Standard for HIV-1 RNA nucleic acid amplification techniques (NAT) in Taiwan | - |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.jviromet.2013.04.002 | - |
dc.relation.pages | 122-127 | - |
dc.relation.journalvolume | 191 | - |
dc.relation.journalissue | 2 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Biotechnology | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Genome and Systems Biology Degree Program | - |
crisitem.author.orcid | 0000-0001-8777-5818 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Life Science | - |
顯示於: | 生物科技研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。