https://scholars.lib.ntu.edu.tw/handle/123456789/384512
Title: | Porcine circovirus type 2 decreases the infection and replication of attenuated classical swine fever virus in porcine alveolar macrophages | Authors: | Huang, Yu-Liang VICTOR FEI PANG Deng, Ming-Chung CHIA-YI CHANG CHIAN-REN JENG |
Keywords: | Alveolar macrophage; Classical swine fever virus; Lapinized Philippines Coronel; Porcine circovirus type 2 | Issue Date: | 2014 | Journal Volume: | 96 | Journal Issue: | 1 | Start page/Pages: | 187-195 | Source: | Research in Veterinary Science | Abstract: | Recently, it has been noted that porcine circovirus type 2 (PCV2) infection adversely affects the protective efficacy of Lapinized Philippines Coronel (LPC) vaccine, an attenuated strain of classical swine fever virus (CSFV), in pigs. In order to investigate the possible mechanisms of the PCV2-derived interference, an in vitro model was established to study the interaction of LPC virus (LPCV) and PCV2 in porcine alveolar macrophages (AMs). The results showed that PCV2 reduced the LPCV infection in AMs and the levels of PCV2-derived interference were dose-dependent. The PCV2-derived interference also reduced the replication level of LPCV in AMs. The full-length PCV2 DNA and its fragment DNA C9 CpG-ODN were involved in the reduction of LPCV infection in AMs, whereas UV-inactivated PCV2 was not. In addition, a moderate negative correlation between the LPCV antigen-containing rate and IFN-γ production was observed, and had a dose-dependent trend with the level of PCV2-inoculation. The results of the present study may partially explain how PCV2 infection interferes with the efficacy of LPC vaccine. ? 2013 Elsevier Ltd. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84892516532&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/384512 |
DOI: | 10.1016/j.rvsc.2013.11.020 | SDG/Keyword: | alpha interferon; CpG oligodeoxynucleotide; gamma interferon; genomic DNA; interleukin 10; interleukin 8; Lapinized Philippines Coronel vaccine; transforming growth factor beta1; tumor necrosis factor alpha; unclassified drug; virus antigen; virus DNA; virus vaccine; animal cell; animal experiment; article; Circovirus; classical swine fever; concentration response; controlled study; correlational study; cytokine production; drug efficacy; in vitro study; lung alveolus macrophage; nonhuman; organismal interaction; Pestivirus; porcine circovirus type 2; sequence analysis; swine; ultraviolet radiation; vaccination; virus genome; virus inactivation; virus interference; virus replication; Classical swine fever virus; Porcine circovirus 2; Suidae; Sus; Alveolar macrophage; Classical swine fever virus; Lapinized Philippines Coronel; Porcine circovirus type 2; Animals; Circoviridae Infections; Circovirus; Classical Swine Fever; Classical swine fever virus; Cytokines; Dose-Response Relationship, Immunologic; Macrophages, Alveolar; Oligodeoxyribonucleotides; Real-Time Polymerase Chain Reaction; RNA, Viral; Specific Pathogen-Free Organisms; Swine; Viral Vaccines; Virus Replication |
Appears in Collections: | 分子暨比較病理生物學研究所 |
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