https://scholars.lib.ntu.edu.tw/handle/123456789/385552
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | MONG-HSUN TSAI | en_US |
dc.contributor.author | ERIC YAO-YU CHUANG | en_US |
dc.creator | MONG-HSUN TSAI;ERIC YAO-YU CHUANG | - |
dc.date.accessioned | 2018-09-10T14:54:47Z | - |
dc.date.available | 2018-09-10T14:54:47Z | - |
dc.date.issued | 2014-07 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/10.1111/1751-7915.12140/abstract | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/385552 | - |
dc.description.abstract | The genotyping of the hepatitis C virus (HCV) plays an important role in the treatment of HCV because genotype determination has recently been incorporated into the treatment guidelines for HCV infections. Most current genotyping methods are unable to detect mixed genotypes from two or more HCV infections. We therefore developed a multiplex genotyping assay to determine HCV genotypes using a bead array. Synthetic plasmids, genotype panels and standards were used to verify the target-specific primer (TSP) design in the assay, and the results indicated that discrimination efforts using 10 TSPs in a single reaction were extremely successful. Thirty-five specimens were then tested to evaluate the assay performance, and the results were highly consistent with those of direct sequencing, supporting the reliability of the assay. Moreover, the results from samples with mixed HCV genotypes revealed that the method is capable of detecting two different genotypes within a sample. Furthermore, the specificity evaluation results suggested that the assay could correctly identify HCV in HCV/human immunodeficiency virus (HIV) co-infected patients. This genotyping platform enables the simultaneous detection and identification of more than one genotype in a same sample and is able to test 96 samples simultaneously. It could therefore provide a rapid, efficient and reliable method of determining HCV genotypes in the future. This genotyping platform enables the simultaneous detection and identification of more than one genotype in a same sample and is able to test 96 samples simultaneously. It could therefore provide a rapid, efficient, and reliable method of determining HCV genotypes in the future. ? 2014 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. | - |
dc.language | en | en |
dc.relation.ispartof | Microbial Biotechnology | en_US |
dc.source | AH | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Article; blood sampling; chemical analysis; genotype; Hepatitis A virus; Hepatitis B virus; Hepatitis C virus; human; Human immunodeficiency virus; Human parvovirus B19; plasmid; reliability; sensitivity and specificity; suspension bead array; classification; devices; evaluation study; genetics; genotype; Hepacivirus; hepatitis C; isolation and purification; multiplex polymerase chain reaction; procedures; single nucleotide polymorphism; virology; Hepatitis C virus; primer DNA; DNA Primers; Genotype; Hepacivirus; Hepatitis C; Humans; Multiplex Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Sensitivity and Specificity | - |
dc.title | A reliable multiplex genotyping assay for HCV using a suspension bead array. | - |
dc.type | journal article | en |
dc.identifier.doi | 10.1111/1751-7915.12140 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Biotechnology | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Genome and Systems Biology Degree Program | - |
crisitem.author.dept | Electrical Engineering | - |
crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Genome and Systems Biology Degree Program | - |
crisitem.author.orcid | 0000-0001-8777-5818 | - |
crisitem.author.orcid | 0000-0003-2530-0096 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Life Science | - |
顯示於: | 生醫電子與資訊學研究所 |
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