https://scholars.lib.ntu.edu.tw/handle/123456789/388226
Title: | Carbon nanodots prepared from o-phenylenediamine for sensing of Cu2+ ions in cells | Authors: | Vedamalai, M. Periasamy, A.P. Wang, C.-W. Tseng, Y.-T. Ho, L.-C. Shih, C.-C. Chang, H.-T. HUAN-TSUNG CHANG |
Issue Date: | 2014 | Journal Volume: | 6 | Journal Issue: | 21 | Start page/Pages: | 13119-13125 | Source: | Nanoscale | Abstract: | A simple hydrothermal method was applied to prepare carbon nanodots (C dots) from o-phenylenediamine (OPD). The C dots exhibit photoluminescence at 567 nm when excited at 420 nm. In the presence of Cu2+ ions, the colour of C dots changes from yellow to orange, with an increased PL intensity as a result of the formation of Cu(OPD)2 complexes on the surfaces of C dots. The D-band to G-band ratios of C dots in the absence and presence of 80 nM Cu2+ ions are 1.31 and 4.75, respectively. The C dots allow the detection of Cu2+ ions with linearity over a concentration range of 2-80 nM, with a limit of detection of 1.8 nM at a signal-to-noise ratio of 3. The cell viability values of A549, MCF-10A, and MDA-MB-231 cells treated with 3 μg mL-1 of C dots are all greater than 99%, showing their great biocompatibility. Having great water dispersibility, photostability, chemical stability (against NaCl up to 0.5 M), great selectivity, and biocompatibility, the C dots have been employed for the localization of Cu2+ ions in the cancer cells (A549 cells) treated with 10 μM Cu2+ ions. ? 2014 the Partner Organisations. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84907992056&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/388226 |
DOI: | 10.1039/c4nr03213f | SDG/Keyword: | Aromatic compounds; Biocompatibility; Carbon; Cells; Chemical stability; Cytology; Nanodots; Signal to noise ratio; Sodium chloride; Carbon nanodots; Concentration ranges; Hydrothermal methods; Limit of detection; Mda-mb-231 cells; O- phenylenediamine; Photo-stability; Water dispersibility; Ions; 1,2-diaminobenzene; biomaterial; carbon; carbon nanotube; copper; ion; phenylenediamine derivative; sodium chloride; water; cell survival; chemistry; electron; human; nanotechnology; signal noise ratio; transmission electron microscopy; tumor cell line; Biocompatible Materials; Carbon; Cell Line, Tumor; Cell Survival; Copper; Electrons; Humans; Ions; Microscopy, Electron, Transmission; Nanotechnology; Nanotubes, Carbon; Phenylenediamines; Signal-To-Noise Ratio; Sodium Chloride; Water |
Appears in Collections: | 化學系 |
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