https://scholars.lib.ntu.edu.tw/handle/123456789/400738
Title: | DNA Modulates the Interaction of Genetically Engineered DNA-Binding Proteins and Gold Nanoparticles: Diagnosis of High-Risk HPV Infection | Authors: | Ju-Yi Mao CHUN-HUA HSU et al. |
Keywords: | clinical diagnosis; DNA-binding proteins; gene detection; genetic engineering; gold nanoparticles; human papillomavirus | Issue Date: | Dec-2017 | Source: | ACS Applied Materials & Interfaces | Abstract: | Gene detection has an important role in diagnosing several serious diseases and genetic defects in modern clinical medicine. Herein, we report a fast and convenient gene detection method based on the modulation of the interaction between a heat-resistant double-stranded DNA (dsDNA)-binding protein (Sso7d) and gold nanoparticles (Au NPs). We prepared a recombinant Cys-Sso7d, which is Sso7d with an extra cysteine (Cys) residue in the N-terminus, through protein engineering to control the interaction between Sso7d and Au NPs. Cys-Sso7d exhibited a stronger affinity for Au NPs and more easily induced the aggregation of Au NPs than Sso7d. In addition, Cys-Sso7d retained its ability to bind with dsDNA. The aggregation of Au NPs induced by Cys-Sso7d was diminished in the presence of dsDNA, which could be utilized as a transduction mechanism for the detection of the polymerase chain reaction (PCR) products of human papillomavirus (HPV) gene fragments (HPV types 16 and 18). The Cys-Sso7d/Au NP probe could detect as few as 1 copy of the HPV gene. The sensitivity and specificity of the Cys-Sso7d/Au NP probe for Pap smear clinical specimens (n = 52) for HPV 16 and HPV 18 detection were 85.7%/100.0% and 85.7%/91.7%, respectively. Our results demonstrate that the Cys-Sso7d/Au NP probe can be used to diagnose high-risk HPV types in Pap smear samples with high sensitivity, specificity, and accuracy. ? 2017 American Chemical Society. |
URI: | https://doi.org/10.1021/acsami.7b13873 http://scholars.lib.ntu.edu.tw/handle/123456789/400738 |
DOI: | 10.1021/acsami.7b13873 | SDG/Keyword: | Amino acids; Bins; Diagnosis; DNA; Fiber optic sensors; Genes; Genetic engineering; Gold; Medicine; Metal nanoparticles; Nanoparticles; Polymerase chain reaction; Probes; Proteins; Recombinant proteins; Clinical diagnosis; DNA-binding protein; Gene detection; Gold Nanoparticles; Human papillomavirus; Gold compounds; DNA; DNA binding protein; gold; metal nanoparticle; female; human; papillomavirus infection; DNA; DNA-Binding Proteins; Female; Gold; Humans; Metal Nanoparticles; Papillomavirus Infections |
Appears in Collections: | 農業化學系 |
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