https://scholars.lib.ntu.edu.tw/handle/123456789/402414
標題: | Analysis of bioactive constituents from the leaves of Amorpha fruticosa L. | 作者: | Cui, X. Guo, J. Lai, C.-S. Pan, M.-H. Ma, Z. Guo, S. Liu, Q. Zhang, L. Ho, C.-T. MIN-HSIUNG PAN |
公開日期: | 2017 | 卷: | 25 | 期: | 4 | 起(迄)頁: | 992-999 | 來源出版物: | Journal of Food and Drug Analysis | 摘要: | Amorpha fruticosa L. is a Chinese folk medicine and rich in polyphenols. Fifteen known compounds were isolated and identified from the leaves of A. fruticosa L. They are tephrosin (1), 6a,12a-dehydrodeguelin (2), vitexin (3), afrormosin-7-O-β-D-glucopyranoside (4), 2?-O-α-L-rhamnopyranosyl isovitexin (5), rutin (6), chrysoeriol (7), 7-O-methylluteolin (8), trans-p-coumaric acid (9), 2-benzyl-4,6-dihydroxybenzoic acid-4-O-β-D-glucopyranoside (10), formononetin (11), quercetin (12), apigenin (13), β-sitosterol (14), and β-daucosterol (15). Compounds 3, 4, 5, and 7–9 were isolated from A. fruticosa L. for the first time. Cytotoxicity of individual compounds 3–10 and 90% ethanol extract against human cancer cell lines HCT116 and HepG2 were reported. The results suggested that compounds 7 and 8, and the crude extract exhibited inhibitory effects on human cancer cell line HCT116, at concentrations of 100 μg/mL, 5 μg/mL, and 25 μg/mL at <60% of cell viability rate, respectively. In addition, a valid high-performance liquid chromatography diode array detector method was established to quantitatively analyze compounds 1–12 in the leaves of A. fruticosa L., which was harvested at three different stages of maturity from May 20 to August 10, 2014. The results demonstrated that contents were greatly influenced by the maturity. Total amounts of the analytical constituents gradually increased from May 20 to August 10, with the values ranging from 10.86 mg/g to 18.84 mg/g, whereas bioactive compounds 7 and 8 presented the opposite variation trend. The results of this study may provide data for further study and comprehensive utilization of A. fruticosa L. resource. ? 2016 |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-85008145760&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/402414 |
DOI: | 10.1016/j.jfda.2016.10.006 | SDG/關鍵字: | 2 benzyl 4,6 dihydroxybenzoic acid 4 o beta dextro glucopyranoside; 2'' o alpha levo rhamnopyranosyl isovitexin; 6a,12a dehydrodeguelin; 7 o methylluteolin; afrormosin 7 o beta dextro glucopyranoside; alcohol; amorpha fruticosa extract; apigenin; beta daucosterol; chrysoeriol; cytotoxic agent; formononetin; garcinol; herbaceous agent; methanol; para coumaric acid; plant extract; quercetin; rutoside; shogaol; sitosterol; tephrosin; unclassified drug; vitexin; plant extract; Amorpha fruticosa; Article; biochemical composition; carbon nuclear magnetic resonance; cell viability; concentration response; controlled study; deciduous plant; developmental stage; drug cytotoxicity; drug isolation; HCT 116 cell line; Hep-G2 cell line; high performance liquid chromatography; human; human cell; legume; limit of detection; limit of quantitation; maturity; measurement accuracy; measurement precision; measurement repeatability; nonhuman; perennial plant; phytochemistry; plant development; plant leaf; proton nuclear magnetic resonance; quantitative analysis; structure activity relation; thin layer chromatography; ultraviolet spectroscopy; validation study; cell survival; chemistry; drug effects; Fabaceae; isolation and purification; tumor cell line; Cell Line, Tumor; Cell Survival; Fabaceae; Humans; Plant Extracts; Plant Leaves |
顯示於: | 食品科技研究所 |
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