https://scholars.lib.ntu.edu.tw/handle/123456789/403269
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Yung-Chieh Chan | en_US |
dc.contributor.author | Ming-Hsien Chan | en_US |
dc.contributor.author | Chieh-Wei Chen | en_US |
dc.contributor.author | RU-SHI LIU | en_US |
dc.contributor.author | Michael Hsiao | en_US |
dc.contributor.author | Din Ping Tsai | en_US |
dc.creator | Din Ping Tsai;Michael Hsiao;Chieh-Wei Chen;Ming-Hsien Chan;Yung-Chieh Chan;RU-SHI LIU | - |
dc.date.accessioned | 2018-12-19T07:22:33Z | - |
dc.date.available | 2018-12-19T07:22:33Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/403269 | - |
dc.description.abstract | The matrix metalloproteinases (MMPs) are well-known mediators that are activated in tumor progression. MMP2 is a kind of gelatinase in extracellular matrix remodeling and cancer metastasis processes. MMP2 secretion increased in many types of cancer diseases, and its abnormal expression is associated with a poor prognosis. We fabricated a nanocomposite that sensed MMP2 expression by a red and blue light change. This nanocomposite consisted of an upconversion nanoparticle (UCNP), MMP2-sensitive peptide, and CuInS2/ZnS quantum dot (CIS/ZnS QD). An UCNP is composed of NaYF4:Tm/Yb@NaYF4:Nd/Yb, which has multiple emissions at UV/blue-visible wavelengths under 808 nm laser excitation. The conjugated CIS/ZnS QD showed the red-visible fluorescence though the FRET process. The two fluorophores were connected by a MMP2-sensitive peptide to form a novel MMP2 biosensor, named UCNP@p-QD. UCNP@p-QD was highly biocompatible according to cell viability assay. The FRET-based biosensor was employed in the MMP2 determination in vitro and in vivo. Furthermore, it was administrated into the tumor-bearing mouse to check MMP2 expression. UCNP@p-QD could be a promising tool for biological study and biomedical application. In this study, we demonstrated that the CIS/ZnS QD improved the upconversion intensity through a near-infrared-induced FRET process. This nanocomposite has the advantage of light penetration, excellent biocompatibility, and high sensitivity to sense MMP2. The near-infrared-induced composites are a potential inspiration for use in biomedical applications. ? 2018 American Chemical Society. | - |
dc.relation.ispartof | ACS Omega | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | Near-Infrared-Activated Fluorescence Resonance Energy Transfer-Based Nanocomposite to Sense MMP2-Overexpressing Oral Cancer Cells | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1021/acsomega.7b01494 | - |
dc.identifier.scopus | 2-s2.0-85054389525 | - |
dc.identifier.isi | WOS:000427936300031 | - |
dc.identifier.url | https://doi.org/10.1021/acsomega.7b01494 | - |
dc.identifier.external | 41476991 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Chemistry | - |
crisitem.author.orcid | 0000-0002-1291-9052 | - |
crisitem.author.parentorg | College of Science | - |
顯示於: | 化學系 |
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