https://scholars.lib.ntu.edu.tw/handle/123456789/404806
標題: | Synthetic virus-like particles prepared via protein corona formation enable effective vaccination in an avian model of coronavirus infection | 作者: | HUI-WEN CHEN Huang, C.-Y. Lin SY Fang ZS Hsu CH Lin JC Chen YI Yao BY Hu, Che-Ming J. |
關鍵字: | Protein corona; Virus-like particles; Gold nanoparticles; Coronavirus; Infectious bronchitis virus; Spike proteins | 公開日期: | 2016 | 出版社: | ELSEVIER SCI LTD | 卷: | 106 | 來源出版物: | Biomaterials | 摘要: | © 2016 Elsevier Ltd The ongoing battle against current and rising viral infectious threats has prompted increasing effort in the development of vaccine technology. A major thrust in vaccine research focuses on developing formulations with virus-like features towards enhancing antigen presentation and immune processing. Herein, a facile approach to formulate synthetic virus-like particles (sVLPs) is demonstrated by exploiting the phenomenon of protein corona formation induced by the high-energy surfaces of synthetic nanoparticles. Using an avian coronavirus spike protein as a model antigen, sVLPs were prepared by incubating 100 nm gold nanoparticles in a solution containing an optimized concentration of viral proteins. Following removal of free proteins, antigen-laden particles were recovered and showed morphological semblance to natural viral particles under nanoparticle tracking analysis and transmission electron microscopy. As compared to inoculation with free proteins, vaccination with the sVLPs showed enhanced lymphatic antigen delivery, stronger antibody titers, increased splenic T-cell response, and reduced infection-associated symptoms in an avian model of coronavirus infection. Comparison to a commercial whole inactivated virus vaccine also showed evidence of superior antiviral protection by the sVLPs. The study demonstrates a simple yet robust method in bridging viral antigens with synthetic nanoparticles for improved vaccine application; it has practical implications in the management of human viral infections as well as in animal agriculture. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/404806 | ISSN: | 01429612 | DOI: | 55276708 http://europepmc.org/abstract/med/27552321 10.1016/j.biomaterials.2016.08.018 |
SDG/關鍵字: | Antigens; Fiber optic sensors; Gold; High resolution transmission electron microscopy; Metal nanoparticles; Nanoparticles; T-cells; Transmission electron microscopy; Vaccines; Viruses; Coronaviruses; Gold Nanoparticles; Protein coronas; Spike protein; Virus-like particles; Proteins; gold nanoparticle; inactivated virus vaccine; protein corona; virus antigen; virus spike protein; coronavirus spike glycoprotein; gold; nanoparticle; protein corona; virus like particle vaccine; animal experiment; animal model; antibody titer; Article; Avian coronavirus; avian model; controlled study; Coronavirus infection; energy; inoculation; microbial morphology; mouse; nonhuman; priority journal; surface property; T lymphocyte; transmission electron microscopy; vaccination; virus like agent; virus particle; animal; bird; chemistry; Coronavirus infection; immunology; procedures; protein engineering; treatment outcome; Animals; Birds; Coronavirus Infections; Gold; Nanoparticles; Protein Corona; Protein Engineering; Spike Glycoprotein, Coronavirus; Treatment Outcome; Vaccines, Virus-Like Particle |
顯示於: | 獸醫學系 |
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