|Title:||A wound-healing assay based on ultraviolet light ablation||Authors:||Wu, Shang Ying
Sun, Yung Shin
|Keywords:||Collective cell migration | Ultraviolet light | Wound-healing assay||Issue Date:||1-Jan-2017||Publisher:||SAGE PUBLICATIONS INC||Journal Volume:||22||Journal Issue:||1||Start page/Pages:||36||Source:||SLAS Technology||Abstract:||
© 2016 Society for Laboratory. Collective cell migration plays important roles in many physiological processes such as embryonic development, tissue repair, and angiogenesis. A “wound” occurs when epithelial cells are lost and/or damaged due to some external factors, and collective cell migration takes place in the following wound-healing process. To study this cellular behavior, various kinds of wound-healing assays are developed. In these assays, a “wound,” or a “cell-free region,” is created in a cell monolayer mechanically, chemically, optically, or electrically. These assays are useful tools in studying the effects of certain physical or chemical stimuli on the wound-healing process. Most of these methods have disadvantages such as creating wounds of different sizes or shapes, yielding batch-to-batch variation, and damaging the coating of the cell culture surface. In this study, we used ultraviolet (UV) lights to selectively kill cells and create a wound out of a cell monolayer. A comparison between the current assay and the traditional scratch assay was made, indicating that these two methods resulted in similar woundhealing rates. The advantages of this UV-created wound-healing assay include fast and easy procedure, high throughput, and no direct contact to cells.
|Appears in Collections:||生物科技研究所|
|A Wound-Healing Assay Based on Ultraviolet Light Ablation.pdf||1.12 MB||Adobe PDF||View/Open|
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