https://scholars.lib.ntu.edu.tw/handle/123456789/406182
標題: | The Roles of Puf6 and Loc1 in 60S Biogenesis Are Interdependent, and Both Are Required for Efficient Accommodation of Rpl43 | 作者: | Yi-Ting Yang Ya-Han Ting Kei-Jen Liang KAI-YIN LO |
關鍵字: | asymmetric mRNA distribution; chaperone; mRNA; precursor ribosomal RNA (pre-rRNA); ribosomal protein; ribosome; ribosome assembly | 公開日期: | 2016 | 出版社: | American Society for Biochemistry {\&} Molecular Biology ({ASBMB}) | 卷: | 291 | 期: | 37 | 起(迄)頁: | 19312 | 來源出版物: | Journal of Biological Chemistry | 摘要: | Puf6 and Loc1 have two important functional roles in the cells, asymmetric mRNA distribution and ribosome biogenesis. Puf6 and Loc1 are localized predominantly in the nucleolus. They bind ASH1 mRNA, repress its translation, and facilitate the transport to the daughter cells. Asymmetric mRNA distribution is important for cell differentiation. Besides their roles in mRNA localization, Puf6 and Loc1 have been shown to be involved in 60S biogenesis. In puf6Δ or loc1Δ cells, pre-rRNA processing and 60S export are impaired and 60S subunits are underaccumulated. The functional studies of Puf6 and Loc1 have been focused on ASH1 mRNA pathway, but their roles in 60S biogenesis are still not clear. In this study, we found that Puf6 and Loc1 interact directly with each other and both proteins interact with the ribosomal protein Rpl43 (L43e). Notably, the roles of Puf6 and Loc1 in 60S biogenesis are interdependent, and both are required for efficient accommodation of Rpl43. Loc1 is further required to maintain the protein level of Rpl43. Additionally, the recruitment of Rpl43 is required for the release of Puf6 and Loc1. We propose that Puf6 and Loc1 facilitate Rpl43 loading and are sequentially released from 60S after incorporation of Rpl43 into ribosomes in yeast. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/406182 | ISSN: | 0021-9258 | DOI: | 10.1074/jbc.M116.732800 https://doi.org/10.1074%2Fjbc.m116.732800 25656574 |
顯示於: | 農業化學系 |
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