https://scholars.lib.ntu.edu.tw/handle/123456789/406742
標題: | Efficient and stable enzyme immobilization in a block copolypeptide vesicle-templated biomimetic silica support | 作者: | Lai J.-K. Chuang T.-H. Jan J.-S. Wang S.S.S. |
關鍵字: | Biomimetic;Block copolypeptide;Enzyme immobilization;Nanocomposites;Silica | 公開日期: | 2010 | 卷: | 80 | 期: | 1 | 起(迄)頁: | 51-58 | 來源出版物: | Colloids and Surfaces B: Biointerfaces | 摘要: | We report the immobilization of a model enzyme, papain, within silica matrices by combining vesiclization of poly-l-lysine-b-polyglycine block copolypeptides with following silica mineralization. Our novel strategy utilizes block polypeptide vesicles to induce the condensation of orthosilicic acid while trapping an enzyme within and between vesicles. The polypeptide mediated silica-immobilized enzyme exhibits enhanced pH and thermal stability and reusability, comparing with the free and vesicle encapsulated enzyme. The enhanced enzymatic activity in the immobilized enzyme is due to the confinement of the enzyme in the polypeptide mediated silica matrices. Kinetic analysis shows that the enzyme functionality is determined by the structure and property of silica/polypeptide matrices. The proposed novel strategy provides an alternative route for the synthesis of a broad range of functional bionanocomposites entrapped within silica nanostructures. ? 2010 Elsevier B.V. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/406742 | ISSN: | 09277765 | DOI: | 10.1016/j.colsurfb.2010.05.030 |
顯示於: | 化學工程學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。