https://scholars.lib.ntu.edu.tw/handle/123456789/407958
標題: | Proteomic analysis of sulfur-nitrogen-carbon removal by Pseudomonas sp. C27 under micro-aeration condition | 作者: | Guo H. Chen C. Lee D.-J. Wang A. Ren N. |
關鍵字: | Denitrifying sulfide removal (DSR);Micro-aerobic stress;Proteomics;Pseudomonas sp. C27 | 公開日期: | 2014 | 卷: | 56 | 起(迄)頁: | 20-27 | 來源出版物: | Enzyme and Microbial Technology | 摘要: | Pseudomonas sp. C27 is a facultative autotrophic bacterium (FAB) that can effectively conduct mixotrophic and heterotrophic denitrifying sulfide removal (DSR) reactions under anaerobic condition using organic matters and sulfide as electron donors. Micro-aeration was proposed to enhance DSR reaction by FAB; however, there is no experimental proof on the effects of micro-aeration on capacity of denitrifying sulfide removal of FAB on proteomic levels. The proteome in total C27 cell extracts was observed by two-dimensional gel electrophoresis. Differentially expressed protein spots and specifically expressed protein spots were identified by MALDI TOF/TOF MS. We identified 55 microaerobic-responsive protein spots, representing 55 unique proteins. Hierarchical clustering analysis revealed that 75% of the proteins were up-regulated, and 5% of the proteins were specifically expressed under micro-aerobic conditions. These enzymes were mainly involved in membrane transport, protein folding and metabolism. The noted expression changes of the microaerobic-responsive proteins suggests that C27 strain has a highly efficient enzyme system to conduct DSR reactions under micro-aerobic condition. Additionally, micro-aeration can increase the rates of protein synthesis and cell growth, and enhance cell defensive system of the strain. ? 2013 Elsevier Inc. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/407958 | ISSN: | 01410229 | DOI: | 10.1016/j.enzmictec.2013.12.013 |
顯示於: | 化學工程學系 |
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