https://scholars.lib.ntu.edu.tw/handle/123456789/407977
標題: | Sulfur-nitrogen-carbon removal of Pseudomonas sp. C27 under sulfide stress | 作者: | Guo H. Chen C. Lee D.-J. Wang A. Ren N. |
關鍵字: | Anaerobic denitrification;Proteomics;Pseudomonas sp. C27;Sulfide | 公開日期: | 2013 | 卷: | 53 | 期: | 1 | 起(迄)頁: | 6-12 | 來源出版物: | Enzyme and Microbial Technology | 摘要: | Pseudomonas sp. C27 is a facultative autotrophic bacterium that can effectively conduct mixotrophic and heterotrophic denitrification reactions using organic matters and sulfide as electron donors. There is no experimental confirmation on proteomic levels the pure C27 strain can have the capability to simultaneous removal of sulfide, nitrate and organic carbon from waters. The proteome in total C27 cell extracts was observed by two-dimensional gel electrophoresis. The 160mg/L sulfide up-regulated or specifically expressed succinate dehydrogenase, iron-sulfur protein, oxidoreductase, serine hydroxymethyltransferase, and iron superoxide dismutase for sulfide metabolism, 2-oxoglutarate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, NAD+-dependent aldehyde dehydrogenase, malate dehydrogenase and succinate dehydrogenase for carbon metabolism, and nitrous-oxide reductase and respiratory nitrate reductase for nitrogen metabolism. The study confirmed that the C27 strain has an effective enzyme system to conduct denitrifying sulfide removal reactions. Also, sulfide stress can enhance energy consumption rate and rates of nitrate reduction and sulfide oxidation by C27. Conversely, sulfide stress repressed the sulfate-reducing power of C27, evidenced by down-regulation or specific un-expression of sulfate ABC transporter, periplasmic sulfate-binding protein in the (C+N+S) sample. ? 2013 Elsevier Inc. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/407977 | ISSN: | 01410229 | DOI: | 10.1016/j.enzmictec.2013.04.002 | SDG/關鍵字: | Denitrifying sulfide removal; Glyceraldehyde-3-phosphate dehydrogenase; Heterotrophic denitrification; Proteomics; Pseudomonas sp; Serine hydroxymethyltransferase; Sulfide; Two-dimensional gel electrophoresis; Amino acids; Bacteria; Carbon; Denitrification; Electrophoresis; Energy utilization; Metabolism; Molecular biology; Nitrates; Nitrogen; Physiology; Proteins; Sulfur; Sulfur compounds; Nitrogen removal; ABC transporter; aldehyde dehydrogenase; carbon; glycine hydroxymethyltransferase; iron sulfur protein; iron superoxide dismutase; malate dehydrogenase; nitrate reductase; nitrogen; nitrous oxide; oxidoreductase; oxoglutarate dehydrogenase; succinate dehydrogenase; sulfide; sulfur; bacterial protein; article; bacterial growth; bacterial strain; carbon metabolism; chemical reaction; chemical stress; cytoplasm; denitrifying bacterium; down regulation; energy consumption; nitrogen metabolism; nonhuman; nucleotide sequence; Pseudomonas; Pseudomonas aeruginosa; Pseudomonas fluorescens; Pseudomonas mendocina; Pseudomonas putida; Pseudomonas stutzeri; Pseudomonas syringae; upregulation; anaerobic growth; bioreactor; enzymology; gene expression regulation; genetics; heat shock response; metabolism; physiology; procedures; proteomics; Pseudomonas; sewage; two dimensional gel electrophoresis; Bacteria (microorganisms); Pseudomonas sp.; Anaerobiosis; Bacterial Proteins; Bioreactors; Carbon; Electrophoresis, Gel, Two-Dimensional; Gene Expression Regulation, Bacterial; Heat-Shock Response; Nitrogen; Proteomics; Pseudomonas; Sulfides; Sulfur; Waste Disposal, Fluid |
顯示於: | 化學工程學系 |
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