https://scholars.lib.ntu.edu.tw/handle/123456789/408304
標題: | A highly efficient two-stage cultivation strategy for lutein production using heterotrophic culture of Chlorella sorokiniana MB-1-M12 | 作者: | Chen C.-Y. Lu I.-C. Nagarajan D. Chang C.-H. Ng I.-S. Lee D.-J. Chang J.-S. |
關鍵字: | Chlorella sorokiniana;Heterotrophic growth;Lutein;Microalgae;Two-stage cultivation | 公開日期: | 2018 | 卷: | 253 | 起(迄)頁: | 141-147 | 來源出版物: | Bioresource Technology | 摘要: | A heterotrophic mutant of Chlorella sorokiniana MB-1-M12 was evaluated for its ability to produce lutein using organic carbon and nitrogen sources and without light irradiation. In batch fermentation, the maximal lutein content (3.67 mg lutein/g biomass) and productivity (2.84 mg/L/d) could be obtained when cultivated in BG-11 medium with 7.5 g/L glucose, 0.75 g/L urea, pH 7.5 and a C/N ratio of 10. A novel two-stage cultivation strategy that integrates fed-batch and semi-batch operations was applied to enhance the lutein production performance. When growing MB-1-M12 strain in a 5L fermenter using the optimal operation strategies, the maximum biomass concentration, biomass productivity, lutein content and lutein productivity could reach 25 g/L, 4.88 mg/L/d, 5.88 mg/g and 16.2 mg/L/d, respectively. This high lutein productivity could significantly reduce the cultivation time and the associated costs, indicating the potential of using MB-1-M12 strain for heterotrophic lutein production in commercial scale. ? 2018 Elsevier Ltd |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/408304 | ISSN: | 09608524 | DOI: | 10.1016/j.biortech.2018.01.027 |
顯示於: | 化學工程學系 |
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