https://scholars.lib.ntu.edu.tw/handle/123456789/409845
標題: | Multifunctional and Continuous Gradients of Biointerfaces Based on Dual Reverse Click Reactions | 作者: | Guan Z.-Y. Wu C.-Y. Wu J.-T. Tai C.-H. Yu J. Chen H.-Y. |
關鍵字: | cell proliferation;click reaction;gradient;multifunctional interface;osteogenesis | 公開日期: | 2016 | 卷: | 8 | 期: | 22 | 起(迄)頁: | 13812-13818 | 來源出版物: | ACS Applied Materials and Interfaces | 摘要: | Chemical or biological gradients that are composed of multifunctional and/or multidirectional guidance cues are of fundamental importance for prospective biomaterials and biointerfaces. As a proof of concept, a general modification approach for generating multifunctional and continuous gradients was realized via two controlled and reversed click reactions, namely, thermo-activated thiol-yne and copper-free alkyne and azide click reactions. The cell adhesion property of fibroblasts was guided in a gradient with an enhancement, showing that the PEG molecule and RGD peptide were countercurrently immobilized to form such reversed gradients (with negating of the cell adhesion property). Using the gradient modification protocol to also create countercurrent distributions of FGF-2 and BMP-2 gradients, the demonstration of not only multifunctional but also gradient biointerfacial properties was resolved in time latencies on one surface by showing the manipulation in gradients toward proliferation and osteogenic differentiation for adipose-derived stem cells. ? 2016 American Chemical Society. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/409845 | ISSN: | 19448244 | DOI: | 10.1021/acsami.6b03908 |
顯示於: | 化學工程學系 |
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