https://scholars.lib.ntu.edu.tw/handle/123456789/412530
標題: | Different domains of synaptotagmin control the choice between kiss-and-run and full fusion | 作者: | CHIH-TIEN WANG Lu, Juu Chin Bai, Jihong Chang, Payne Y. Martin, Thomas F.J. Chapman, Edwin R. Jackson, Meyer B. |
公開日期: | 21-八月-2003 | 出版社: | NATURE PUBLISHING GROUP | 卷: | 424 | 期: | 6951 | 起(迄)頁: | 943 | 來源出版物: | Nature | 摘要: | Exocytosis - the release of the contents of a vesicle - proceeds by two mechanisms. Full fusion occurs when the vesicle and plasma membranes merge. Alternatively, in what is termed kiss-and-run, vesicles can release transmitter during transient contacts with the plasma membrane. Little is known at the molecular level about how the choice between these two pathways is regulated. Here we report amperometric recordings of catecholamine efflux through individual fusion pores. Transfection with synaptotagmin (Syt) IV increased the frequency and duration of kiss-and-run events, but left their amplitude unchanged. Endogenous Syt IV, induced by forskolin treatment, had a similar effect. Full fusion was inhibited by mutation of a Ca 2+ ligand in the C 2 A domain of Syt I; kiss-and-run was inhibited by mutation of a homologous Ca 2+ ligand in the C 2 B domain of Syt IV. The Ca 2+ sensitivity for full fusion was 5-fold higher with Syt I than Syt IV, but for kiss-and-run the Ca 2+ sensitivities differed by a factor of only two. Syt thus regulates the choice between full fusion and kiss-and-run, with Ca 2+ binding to the C 2 A and C 2 B domains playing an important role in this choice. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/412530 | ISSN: | 00280836 | DOI: | https://api.elsevier.com/content/abstract/scopus_id/0041858035 10.1038/nature01857 |
顯示於: | 分子與細胞生物學研究所 |
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